Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6241 |
| PDBID: | 8G9W |
| Chains: | HL_E |
| Organism: | Human immunodeficiency virus 1, Mus musculus |
| Method: | EM |
| Resolution (Å): | 4.66 |
| Reference: | 10.1128/jvi.01604-22 |
| Antibody | |
| Antibody: | vFP49.02 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 DS-SOSIP.664 envelope glycoprotein - gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8G9W_H|Chain E[auth H]|vFP49.02 heavy chain|Mus musculus (10090) EVMLVESGGGLVKPGGSLKLSCEASGFSFGFYSLSWVRQTPEKRLEWVATIAGSGVGGQTYYPDSVKGRFTISRDNAKNTLYLQMSSLRSEDTAVFYCARHGEGKYGSNFAYWGQGTTLTVSSASTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCDKGLEVLFQG |
Light Chain: L
Mutation: NULL
| >8G9W_L|Chain F[auth L]|vFP49.02 light chain|Mus musculus (10090) DVVLTQSPATLSVTPGDSVSLSCRASQTISDNLHWYLQKSHESPRLLIKYSSQSISGIPSRFSGSGSGTDFTLNINSVETEDFGMYFCQQTNSWPLTFGAGTKLELKRTDAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >8G9W_E|Chain C[auth E], D[auth G], H[auth P]|Envelope glycoprotein gp120|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSACTQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQCMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY26 PHE27 SER28 PHE31 ALA74 ASN76 GLU97 GLY98 L: SER56 GLY57 E: ASN80 GLN82 GLU83 ILE84 HIS85 GLU87 LYS229 LYS231 GLU268 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
