Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6226 |
| PDBID: | 8G3Z |
| Chains: | EF_D |
| Organism: | Homo sapiens, Influenza B virus |
| Method: | EM |
| Resolution (Å): | 2.30 |
| Reference: | 10.1038/s41586-023-06136-y |
| Antibody | |
| Antibody: | FNI17 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza B virus Massachusetts/02/2012 Yamagata neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P27907; P03474; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >8G3Z_E|Chain A[auth E], C[auth G], E[auth H], G[auth J]|FNI17 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKRPGSSVKVSCKPSGGTFSNNVISWVRQAPGQGLEWMGGIIPTSGIANYAQKFQGRVAIIADKSTSTVYMALSSLRSEDSAVYFCARARSDYFNRDLGWEDYYFENWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: F
Mutation: NULL
| >8G3Z_F|Chain B[auth F], D[auth I], F[auth K], H[auth L]|FNI17 Fab light chain|Homo sapiens (9606) EIVMTQSPATLSVSPGERATLSCRASQSVGSSLVWYQQKPGQAPRLLIYGASTRATGVPARFSGSGSGTEFTLTISSLQSEDFAVYYCQHYNNWPPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >8G3Z_D|Chain I[auth A], J[auth B], K[auth C], L[auth D]|Neuraminidase|Influenza B virus (11520) METDTLLLWVLLLWVPGSTGDHHHHHHGSGLNDIFEAQKIEWHEGSIINETADDIVYRLTVIIDDRYESLKNLITLRADRLEMIINDNVSTILASGLVPRGSGSLPEPEWTYPRLSCPGSTFQKALLISPHRFGEIKGNSAPLIIREPFIACGPTECKHFALTHYAAQPGGYYNGTREDRNKLRHLISVKLGKIPTVENSIFHMAAWSGSACHDGKEWTYIGVDGPDSNALLKIKYGEAYTDTYHSYAKNILRTQESACNCIGGDCYLMITDGPASGVSECRFLKIREGRIIKEIFPTGRVKHTEECTCGFASNKTIECACRDNRYTAKRPFVKLNVETDTAEIRLMCTETYLDTPRPNDGSITGPCESDGDKGSGGIKGGFVHQRMASKIGRWYSRTMSKTKRMGMGLYVKYDGDPWTDSEALALSGVMVSMEEPGWYSFGFEIKDKKCDVPCIGIEMVHDGGKTTWHSAATAIYCLMGSGQLLWDTVTGVDMAL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ILE52 SER55 GLY56 ILE57 ALA58 ASN59 TYR60 GLN62 GLN65 ASP102 TYR103 PHE104 ASN105 ARG106 ASP107 LEU108 GLU111 F: GLN27 SER28 TRP94 PRO95 PRO96 D: ARG116 GLU117 ARG147 GLU148 ASP149 ARG150 TRP177 SER178 PRO196 ASP197 SER198 LYS219 ASN220 ILE221 ARG223 GLU226 PRO244 ALA245 SER246 GLY247 VAL248 GLU275 GLU276 ARG292 ASN294 ARG295 TYR296 LYS343 SER345 GLY346 GLY347 LYS373 ARG374 TRP408 TYR409 GLY434 LYS435 HIS439 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
