Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6224 |
| PDBID: | 8G3R |
| Chains: | CB_G |
| Organism: | Influenza A virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.30 |
| Reference: | 10.1038/s41586-023-06136-y |
| Antibody | |
| Antibody: | FNI17 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus Tanzania/205/2010 H3N2 neuraminidase |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >8G3R_C|Chain D[auth C]|FNI17 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKRPGSSVKVSCKPSGGTFSNNVISWVRQAPGQGLEWMGGIIPTSGIANYAQKFQGRVAIIADKSTSTVYMALSSLRSEDSAVYFCARARSDYFNRDLGWEDYYFENWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: B
Mutation: NULL
| >8G3R_B|Chain C[auth B]|FNI17 Fab light chain|Homo sapiens (9606) EIVMTQSPATLSVSPGERATLSCRASQSVGSSLVWYQQKPGQAPRLLIYGASTRATGVPARFSGSGSGTEFTLTISSLQSEDFAVYYCQHYNNWPPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: G
Mutation: S268N/S270T
| >8G3R_G|Chain A[auth E], B[auth F], E[auth G], F[auth H]|Neuraminidase|Influenza A virus (11320) METDTLLLWVLLLWVPGSTGDHHHHHHGSGLNDIFEAQKIEWHEGSIINETADDIVYRLTVIIDDRYESLKNLITLRADRLEMIINDNVSTILASGLVPRGSGSAEYRNWSKPQCDITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPDKCYQFALGQGTTLNNVHSNNTVRDRTPYRTLLMNELGVPFHLGTKQVCIAWSSSSCHDGKAWLHVCITGDDKNATASFIYNGRLVDSVVSWSKEILRTQESECVCINGTCTVVMTDGNATGKADTKILFIEEGKIVHTSTLSGSAQHVEECSCYPRYPGVRCVCRDNWKGSNRPIVDINIKDHSIVSSYVCSGLVGDTPRKNDSSSSSHCLDPNNEEGGHGVKGWAFDDGNDVWMGRTINETSRLGYETFKVIEGWSNPKSKLQINRQVIVDRGNRSGYSGIFSVEGKSCINRCFYVELIRGRKEETEVLWTSNSIVVFCGTSGTYGTGSWPDGADLNLMPI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN27 SER28 SER32 ASN93 TRP94 PRO95 C: ASN31 ILE52 SER55 GLY56 ILE57 ALA58 ASN59 GLN62 GLN65 ASP102 TYR103 PHE104 ASN105 ARG106 ASP107 LEU108 GLU111 G: ARG118 GLU119 VAL149 ARG150 ASP151 ARG152 TRP178 SER179 ASP198 LYS199 LYS220 GLU221 ILE222 ARG224 GLU227 ASN245 GLU276 GLU277 ARG292 ASN294 TRP295 LYS296 GLU344 GLY346 HIS347 THR369 ARG371 TYR406 LYS431 GLU432 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
