Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6196 |
| PDBID: | 8FGX |
| Chains: | BA_C |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.62 |
| Reference: | 10.1124/jpet.123.001740 |
| Antibody | |
| Antibody: | STAR-0215 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Active human plasma kallikrein light chain |
| Antigen mutation: | No |
| Durg Target: | P03952 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >8FGX_B|Chain B|STAR-0215 Heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGYAFSSYWMNWVRQAPGQGLEWIGQIYPGDDDTNYNAKFQGRVTITVDKSTTTAYMELSSLRSEDTAVYFCAGSLMVTTGAPFDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: A
Mutation: NULL
| >8FGX_A|Chain A|STAR-0215 Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCKASQDVGIAVAWYQQKPGKAPKFLIYYASHRGWGVPDRFSGSGSGTDFTLTISSLQPEDFATYFCQQYRSYPLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >8FGX_C|Chain C|Plasma kallikrein light chain|Homo sapiens (9606) GCLTQLYENAFFRGGDVASMYTPNAQYCQMRCTFHPRCLLFSFLPASSINDMEKRFGCFLKDSVTGTLPKVHRTGAVSGHSLKQCGHQISACHRDIYKGVDMRGVNFNVSKVSSVEECQKRCTNNIRCQFFSYATQTFHKAEYRNNCLLKYSPGGTPTAIKVLSNVESGFSLKPCALSEIGCHMNIFQHLAFSDVDVARVLTPDAFVCRTICTYHPNCLFFTFYTNVWKIESQRNVCLLKTSESGTPSSSTPQENTISGYSLLTCKRTLPEPCHSKIYPGVDFGGEELNVTFVKGVNVCQETCTKMIRCQFFTYSLLPEDCKEEKCKCFLRLSMDGSPTRIAYGTQGSSGYSLRLCNTGDNSVCTTKTSTRIVGGTNSSWGEWPWQVSLQVKLTAQRHLCGGSLIGHQWVLTAAHCFDGLPLQDVWRIYSGILNLSDITKDTPFSQIKEIIIHQNYKVSEGNHDIALIKLQAPLNYTEFQKPICLPSKGDTSTIYTNCWVTGWGFSKEKGEIQNILQKVNIPLVTNEECQKRYQDYKITQRMVCAGYKEGGKDACKGDSGGPLVCKHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILEKTQSSDGKAQMQSPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ILE31 ALA32 TYR49 TYR50 HIS53 TRP56 ARG92 B: VAL2 LEU4 TYR27 ALA28 SER30 SER31 TYR32 TRP33 TYR52 GLY54 ASP55 ASP57 GLY98 SER99 LEU100 MET101 VAL102 THR103 ASP109 TYR110 C: ILE391 VAL392 GLY393 GLY394 ASN396 SER397 SER398 TRP399 GLY400 GLU401 TRP402 TRP404 GLU497 PHE498 THR515 ASN516 TRP518 LYS537 ASN539 LYS585 HIS586 ASN587 GLY588 TRP590 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
