Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6193 |
| PDBID: | 8FE4 |
| Chains: | HL_B |
| Organism: | dengue virus type 2, Mus musculus |
| Method: | EM |
| Resolution (Å): | 9.80 |
| Reference: | 10.1073/pnas.2218899120 |
| Antibody | |
| Antibody: | prM13 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus type 2 (DENV2) flavivirus premembrane (prM) protein |
| Antigen mutation: | No |
| Durg Target: | P12823 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8FE4_H|Chain C[auth H], G[auth M], K[auth O]|prM13 Fab Heavy Chain|Mus musculus (10090) AAQLQESGPELVKPGASVKISCKASGYTFTDYFINWVRQSHGKSLEWIGDFYPNNRGPTYNQKFEGKATLTVDKSSSTAYMELRSLTSDDSAVYYCARGLWDAWLSYWGQGTLVTVSAAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVPI |
Light Chain: L
Mutation: NULL
| >8FE4_L|Chain D[auth L], H[auth N], L[auth P]|prM13 Fab Light Chain|Mus musculus (10090) AIVATQSPAIMSASPGEKVTISCSASSSVSYMYWYQQKPGSSPKPWIYRTSNLASGVPTRFSGSGSGTSYSFTISSMEAEDAATYYCQQYHNYPRTFGGGTKAKSARADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >8FE4_B|Chain B, F[auth D], J[auth F]|prM protein|Dengue virus type 2 (11060) FHLTTRNGEPHMIVSRQEKGKSLLFKTEDGVNMCTLMAMDLGELCEDTITYKCPLLRQNEPEDIDCWCNSTSTWVTYGTCT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 THR28 THR30 ASP31 TYR32 TYR52 ASN54 ASN55 LEU100 TRP101 ASP102 TRP104 SER106 L: TYR31 TYR90 HIS91 B: PHE1 HIS2 LEU3 THR5 ASN7 GLY8 PRO10 PHE25 LYS26 THR27 GLU28 THR71 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
