Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6191 |
| PDBID: | 8FE3 |
| Chains: | MN_D |
| Organism: | Mus musculus, dengue virus type 2 |
| Method: | EM |
| Resolution (Å): | 10.20 |
| Reference: | 10.1073/pnas.2218899120 |
| Antibody | |
| Antibody: | prM12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus type 2 (DENV2) flavivirus premembrane (prM) protein |
| Antigen mutation: | No |
| Durg Target: | P12823 |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >8FE3_M|Chain A[auth H], G[auth M], I[auth O]|prM12 Fab Heavy Chain|Mus musculus (10090) EVQLQESGPELVKPGTSVKMSCKASGYTFTDYNIHWVKQSHGKSLEWIGYINPNNGGNKYTQKFEGRATLTVGKSSSTAYMELRSLTSEDSAVYYCARSPMYFYYDGSYYFDYWGQGTSLSVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVPI |
Light Chain: N
Mutation: NULL
| >8FE3_N|Chain B[auth L], H[auth N], J[auth P]|prM12 Fab Light Chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISYRASKSVSTSGYSYMHWNQQKPGQPPRLLIYLVSNLESGVPARFSGSGSGTDFTLNIHPVEEEDAATYYCQHIRELTFGAAPSAAARADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >8FE3_D|Chain D[auth B], F[auth D], L[auth F]|prM protein|Dengue virus type 2 (11060) FHLTTRNGEPHMIVSRQEKGKSLLFKTEDGVNMCTLMAMDLGELCEDTITYKCPLLRQNEPEDIDCWCNSTSTWVTYGTCT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: THR30 ASP31 TYR32 ASN33 TYR50 ASN54 LYS59 TYR102 PHE103 TYR104 TYR105 ASP106 GLY107 TYR109 N: THR31 SER32 ARG96 GLU97 D: LEU3 THR4 THR5 ASN7 GLY8 GLU9 PRO10 HIS11 LEU24 PHE25 LYS26 THR27 GLU28 ASP29 GLY30 ASN32 THR73 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
