Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6186 |
| PDBID: | 8F6X |
| Chains: | F_A |
| Organism: | human metapneumovirus, Escherichia phage T2, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.25 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | MPE8 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | A structurally designed human metapneumovirus (HMPV) F protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >8F6X_F|Chain D, E, F|MPE8 Single chain variable fragment|Homo sapiens (9606) MELGLRWVFLVAILEGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFSSYSMNWVRQAPGKGLEWVSSISASSSYSDYADSAKGRFTISRDNAKTSLFLQMNSLRAEDTAIYFCARARATGYSSITPYFDIWGQGTLVTVSSGTGGSGGGGSGGGGSGGGASQSVVTQTPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYDNNNRPSGVPDRFSASKSGTSASLAITGLQAEDEADYYCQSYDRNLSGVFGTGTKVTVLGSGENLYFQSGGSGGHHHHHHHHSAWSHPQFEK |
Antigen
Chain: A
Mutation: V84C/A122C/A129C/A167P/A231C/D436C/V440C
| >8F6X_A|Chain A, B, C|Structurally designed HMPV F protein HMPV_v3B_D12_DS454,Fibritin|Human metapneumovirus (162145) MSWKVMIIISLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCTDGPSLIKTELDLTKSALRELKTCSADQGSGGSGATAAAVTAGIAIAKTIRLESEVNAIKGCLKTTNECVSTLGNGVRVLATAVRELKEFVSKNLTSAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSYMPTSAGQIKLMLENRCMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIIKAAPSCSEKDGNYACLLREDQGWYCKNAGSTVYYPNDKDCETRGDHVFCDTAAGINVAEQSRECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLPKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPIKFPECQFNCALDQVFESIENSQALVDQSNKILNSAESAIGGYIPEAPRDGQAYVRKDGEWVLLSTFLGGLVPRGSHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: THR28 SER30 SER31 TYR32 SER52 ALA53 SER54 SER56 TYR57 ASP59 ARG100 ALA101 THR102 GLY103 TYR104 SER105 SER106 ILE107 ILE173 GLY174 ALA175 GLY176 TYR177 LYS212 GLY214 THR215 TYR237 ARG239 A: LEU36 THR41 GLY42 TRP43 ALA120 ILE124 VAL148 VAL155 ARG156 SER232 TYR233 MET234 PRO235 THR236 SER237 ALA238 GLY239 GLN240 ILE241 LYS242 LEU243 ILE275 PHE276 GLY277 VAL278 ILE279 ASP280 THR281 PRO282 LYS312 ASN313 ALA314 GLY315 HIS332 GLU345 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
