Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6183 |
| PDBID: | 8F6O |
| Chains: | AB_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.31 |
| Reference: | 10.1016/j.str.2023.05.011 |
| Antibody | |
| Antibody: | h22B3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Venanprubart(Phase-II) |
| Antigen | |
| Antigen: | Human B and T lymphocyte attenuator (BTLA) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8F6O_A|Chain A|h22B3 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGFSLSSYGVSWVRQAPGQGLEWMGAISYDGITYYASWAKSRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGDYYDDYVYVYALDIWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGHHHHHH |
Light Chain: B
Mutation: NULL
| >8F6O_B|Chain B|h22B3 Fab light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCQASQSISTALAWYQQKPGQAPRLLIYAASTLASGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQGYSSSNLDNVFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >8F6O_C|Chain C|B- and T-lymphocyte attenuator|Homo sapiens (9606) KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMASEFHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER31 ILE51 TYR53 ASP54 GLY55 ILE56 THR57 TYR58 MET69 THR70 TYR100 ASP102 ASP103 TYR104 VAL105 TYR106 B: GLU1 ILE2 GLN27 SER28 SER30 SER67 GLY68 TYR92 SER93 SER95 ASN96 LEU97 C: ASP35 VAL36 GLN37 LEU38 TYR39 ILE40 LYS41 ARG42 LEU74 GLY76 THR77 THR78 CYS79 ARG114 PHE119 GLN120 SER121 ASN122 LEU123 ILE124 GLU125 SER126 HIS127 SER128 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
