Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6182 |
| PDBID: | 8F6L |
| Chains: | AB_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.85 |
| Reference: | 10.1016/j.str.2023.05.011 |
| Antibody | |
| Antibody: | h25F7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human B and T lymphocyte attenuator (BTLA) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8F6L_A|Chain A|h25F7 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGFSLWTYAMNWVRQAPGQGLEWMGIISDDGTTYYATWAKGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDAGAGGVQDYLTLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDGSHHHHHHHH |
Light Chain: B
Mutation: NULL
| >8F6L_B|Chain B|h25F7 Fab light chain|Homo sapiens (9606) DIVMTQSPDSLAVSLGERATINCQASRNIYNFLAWYQQKPGQPPKLLIYSASTLASGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQGSSNSNIDNPFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >8F6L_C|Chain C|B- and T-lymphocyte attenuator|Homo sapiens (9606) KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMASEFHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TRP30 THR31 TYR32 SER52 ASP53 ASP54 THR56 TYR58 ALA99 GLY100 ALA101 GLY102 GLY103 VAL104 ASP106 TYR107 B: ARG27 TYR30 ASN31 PHE32 GLY91 SER92 SER93 SER95 C: GLU55 TYR62 ALA64 ASN65 ARG66 PRO67 HIS68 THR70 LYS81 ARG85 GLN86 THR87 SER88 TRP89 LYS90 GLU91 GLU92 SER96 PHE98 ASN118 GLN120 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
