Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6181 |
| PDBID: | 8F60 |
| Chains: | AB_C |
| Organism: | Oryctolagus cuniculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.64 |
| Reference: | 10.1016/j.str.2023.05.011 |
| Antibody | |
| Antibody: | r23C8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human B and T lymphocyte attenuator (BTLA) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8F60_A|Chain A|r23C8 Fab heavy chain|Oryctolagus cuniculus (9986) QSVEESGGRLVTPGTPLTLTCTASGFDISKYNIQWVRQSPGKGLEYIGFINYGGSAYYASRAKGRFTISKTSTTVDLKIASPTTGDTATYFCARGLSNSDLWGPGTLVTVSLGQPKAPSVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSGTLTNGVRTFPSVRQSSGLYSLSSVVSVTSSSQPVTCNVAHPATNTKVDKTVAPSTCSHHHHHHHH |
Light Chain: B
Mutation: NULL
| >8F60_B|Chain B|r23C8 Fab light chain|Oryctolagus cuniculus (9986) AIDMTQTPSSVSEPVGGTVTIKCQASQSISSWLSWYQQKPGQPPKLLIYRASTLASGIPSRFKGSGSGTEYTLTISDLECADAATYYCQCTYGGVVGSTSDDNPFGGGTEVVVKGDPVAPTVLIFPPAADQVATGTVTIVCVANKYFPDVTVTWEVDGTTQTTGIENSKTPQNSADCTYNLSSTLTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRGDC |
Antigen
Chain: C
Mutation: S17H
| >8F60_C|Chain C|B- and T-lymphocyte attenuator|Homo sapiens (9606) KESCDVQLYIKRQSEHHILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMASEFIDGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: LYS30 TYR31 ASN32 PHE49 ASN51 TYR52 SER55 TYR57 ARG94 GLY95 LEU96 SER97 ASN98 ASP100 B: TRP32 TYR49 ARG50 THR53 SER56 THR91 GLY93 GLY94 VAL95 VAL96 GLY97 SER98 ASP101 C: HIS46 HIS47 ILE48 LEU49 ALA50 GLY51 ASP52 PRO53 PHE54 GLU55 GLU57 GLU103 PRO104 LEU106 PRO107 THR134 ASP135 VAL136 LYS137 SER138 ALA139 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
