Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6180 |
| PDBID: | 8F5N |
| Chains: | HL_A |
| Organism: | Streptococcus pyogenes, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1080/21505594.2023.2180228 |
| Antibody | |
| Antibody: | E3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Streptococcus pyogenes backbone pilins/T-antigens T18.1 N-domain |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8F5N_H|Chain C[auth H]|Mouse-Human Fab heavy chain|Homo sapiens (9606) EVQLQQSGAELVKPGASVKLSCKASGYTFTSFYMYWVKQRPGQGLEWIGGINPSNGGANFNEKFKNKATLTVDKSSSTAYMQLSSLTSEDSAVYYCTRGFYYGHWYFDVWGAGTTVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >8F5N_L|Chain B[auth L]|Mouse-Human Fab light chain|Homo sapiens (9606) GADIVMTQSTSSLSASLGDRVTISCRASQDISNSLNWYQQKPDGTVKLLIHYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQVNTLPLTFGAGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSLPVTKSFNRGEC |
Antigen
Chain: A
Mutation: A272T
| >8F5N_A|Chain A|T18.1 Major pilin backbone protein T-antigen|Streptococcus pyogenes (1314) ETAGVIDGSTLVVKKTFPSYTDDKVLMPKADYTFKVEADDNAKGKTKDGLDIKPGVIDGLENTKTIHYGNSDKTTAKEKSVNFDFANVKFPGVGVYRYTVSEVNGNKAGIAYDSQQWTVDVYVVNREDGGFEAKYIVSTEGGQSDKKPVLFKNFFDTTSLKVTKKVTGNTGEHQRSFSFTLLLTPNECFEKGQVVNILQGGETKKVVIGEEYSFTLKDKESVTLSQLPVGIEYKVTEEDVTKDGYKTSATLKDGDVTDGYNLGDSKTTDKSTDEIVVTNKRD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: HIS55 SER56 VAL91 ASN92 THR93 LEU94 LEU96 H: VAL2 TYR27 THR28 SER31 PHE32 TYR33 ASN52 SER54 ASN55 GLY57 ALA58 ASN59 ARG98 PHE100 TYR101 TYR102 GLY103 HIS104 TRP105 ASP108 A: LYS45 THR46 LYS47 ASP48 GLY49 LEU50 ASP51 LYS53 PRO91 GLY92 VAL93 TYR122 VAL123 VAL124 ASN125 ARG126 GLU127 ASP128 GLY129 PHE131 GLU132 LYS134 TYR135 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
