Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6173 |
| PDBID: | 8EZM |
| Chains: | H_A |
| Organism: | Plasmodium falciparum, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1038/s41541-023-00712-z |
| Antibody | |
| Antibody: | AS01-63 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum 25 kDa ookinete surface antigen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >8EZM_H|Chain B[auth H]|Transmission-reducing antibody AS01-63, Single-chain Fv|Homo sapiens (9606) TGQVQLVESGGGVVQPGRSLRLSCAASGFSFGSHDMSWVRQAPGKGLDWVAVIWYDGSKKYYADSVKGRFTISRDSSKKTLYLQMNTLRAEDTAVYYCARAAYDSRSLDYWGHGTLVTISSGGGGSGGGGSGGGGSGGGGSQPVLTQPPSASASLGASLSLTCTLSSAYSNYSVDWYQQRPGKGPRFVMRVGTGGIVRSKGDGIPDRFSVLASGLNRYLTIQNIQEEDESDYHCGADHGSGSNFLYVFGTGTKVTVLGTKHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >8EZM_A|Chain A|25 kDa ookinete surface antigen|Plasmodium falciparum (5833) TGKVTVDTVCKRGFLIQMSGHLECKCENDLVLVNEETCEEKVLKCDEKTVNKPCGDFSKCIKIDGNPVSYACKCNLGYDMVNNVCIPNECKQVTCGNGKCILDTSNPVKTGVCSCNIGKVPNVQDQNKCSKDGETKCSLKCLKEQETCKAVDGIYKCDCKDGFIIDQESSICTGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: PHE27 SER28 PHE29 GLY30 SER31 ASP33 TYR53 ASP54 SER74 SER75 LYS76 LYS77 ALA100 TYR101 ASP102 SER103 ARG104 TYR170 SER171 ARG188 ARG196 ASP235 GLY237 SER238 A: ILE59 LYS60 ILE61 ASP62 GLY63 ASN64 PRO65 VAL66 SER67 ALA69 CYS70 LYS71 CYS72 ASN73 LEU74 GLY75 TYR76 ASP77 MET78 VAL79 ASN81 ASN86 LYS89 GLN90 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
