Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6170 |
| PDBID: | 8EZK |
| Chains: | H_A |
| Organism: | Plasmodium falciparum, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1038/s41541-023-00712-z |
| Antibody | |
| Antibody: | AS01-04 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum 25 kDa ookinete surface antigen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >8EZK_H|Chain C[auth H], D[auth I]|Transmission-reducing antibody AS01-4, single-chain Fv|Homo sapiens (9606) TGQVQLQESGPGLVKPSETLSLTCTVSGFSISSSYYWDWIRQPPGKGLEWIGNIHHTGATYYNPSLKSRVTISIDTANNQFSLRLRSVTAADAAVYYCARGGMGTTKRGWFDPWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSQSVLTQPPSASGTPGQRVTISCSGSSSNIRVNYVYWYQQIPGTAPKLLIYRNDQRPSGVPDRFSASKSGTSASLAISGLRSQDEADYYCAAWDDSLTGPVFGGGTKLTVLGTKHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >8EZK_A|Chain A, B|25 kDa ookinete surface antigen|Plasmodium falciparum (isolate NF54) (5833) TGKVTVDTVCKRGFLIQMSGHLECKCENDLVLVNEETCEEKVLKCDEKTVNKPCGDFSKCIKIDGNPVSYACKCNLGYDMVNNVCIPNECKQVTCGNGKCILDTSNPVKTGVCSCNIGKVPNVQDQNKCSKDGETKCSLKCLKEQETCKAVDGIYKCDCKDGFIIDQESSICTGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: PHE27 SER28 SER30 SER31 SER32 TYR33 TYR34 HIS53 HIS54 THR55 GLY56 ALA57 THR58 TYR59 MET101 GLY102 THR103 THR104 LYS105 ARG106 SER167 SER168 SER169 ARG172 VAL173 TRP234 ASP236 SER237 LEU238 THR239 A: VAL7 CYS8 LYS9 ARG10 GLY11 GLU25 ASN26 ASP27 LEU28 GLU37 GLU38 LYS39 VAL40 LEU41 LYS42 ASP44 GLU45 LYS46 THR47 LYS50 PRO51 LYS141 GLN165 GLU166 SER167 SER168 ILE169 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
