Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6168 |
| PDBID: | 8EZ7 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Influenza A virus (A/Moscow/10/1999(H3N2)) |
| Method: | EM |
| Resolution (Å): | 2.60 |
| Reference: | 10.1016/j.immuni.2023.10.005 |
| Antibody | |
| Antibody: | 1F04 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus Moscow/10/1999 H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | P06818 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8EZ7_H|Chain A[auth H]|Heavy chain of influenza virus neuraminidase antibody 1F04|Homo sapiens (9606) EVQLVESGGGLVLPGRSLRLSCVASGFDFSSFEMNWVRQAPGTGLEWLSYISGNGDTIYYAESVKGRFTISRDNAKNSLYLQMNGLRAEDTATYYCARQRSVYSTSRSNWSVFLDDWGQGTLVTVS |
Light Chain: L
Mutation: NULL
| >8EZ7_L|Chain B[auth L]|Light chain of influenza virus neuraminidase antibody 1F04|Homo sapiens (9606) EIVLTQSPGTLSLSPGESATLSCRASQSLAPNYLAWFQQQPGQAPRLLIYGASSRAAGIPDRFSGSGSGTDFTLTITRLEPEDFAVYYCQQYGGSPPYTFGQGTKVEIKR |
Antigen
Chain: A
Mutation: NULL
| >8EZ7_A|Chain C[auth A]|Neuraminidase|Influenza A virus (A/Moscow/10/1999(H3N2)) (480019) AEYRNWSKPQCNITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPDKCYQFALGQGTTLNNGHSNDTVHDRTPYRTLLMNELGVPFHLGTKQVCIAWSSSSCHDGKAWLHVCVTGDDENATASFIYNGRLVDSIGSWSKKILRTQESECVCINGTCTVVMTDGSASGKADTKILFIEEGKIVHTSPLSGSAQHVEECSCYPRYPGVRCVCRDNWKGSNRPIVDINVKDYSIVSSYVCSGLVGDTPRKNDSSSSSHCLDPNNEEGGHGVKGWAFDDGNDVWMGRTISEKLRSGYETFKVIEGWSKPNSKLQINRQVIVDRGNRSGYSGIFSVEGKSCINRCFYVELIRGRKQETEVLWTSNSIVVFCGTSGTYGTGSWPDGADINLMPI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLU33 TYR50 ASN53 ASP55 THR56 TYR58 GLN95 SER97 TYR99 L: ILE2 SER27 LEU28 ALA29 PRO30 ASN31 TYR32 SER67 GLY68 GLY92 GLY93 SER94 TYR96 A: HIS264 SER266 PRO267 LEU268 SER269 GLY270 SER271 GLN273 ARG283 ARG288 LYS296 ASP304 ASN306 ASP309 TYR310 SER311 ILE312 VAL313 SER314 SER315 TYR316 CYS318 CYS337 LEU338 ASP339 PRO340 ASN342 ASP355 GLY357 ASN358 TRP383 SER384 PRO386 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
