Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6167 |
| PDBID: | 8EZ3 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Influenza A virus (A/Moscow/10/1999(H3N2)) |
| Method: | EM |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/j.immuni.2023.10.005 |
| Antibody | |
| Antibody: | 3A10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus Moscow/10/1999 H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | P06818 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8EZ3_H|Chain A[auth H]|Heavy chain of influenza virus neuraminidase antibody 3A10|Homo sapiens (9606) QVQLQESGPGLVRPSQTLSLTCTVSGASISDVDFYWTWFRQTPGKGLEWIGYTNSIGNAFYNPSLQSRLTISIDTSDNHFSLRLKSVTAADTAVYYCARSNSNTWSREYFQHWGQGTLVTVSS |
Light Chain: L
Mutation: NULL
| >8EZ3_L|Chain B[auth L]|Light chain of influenza virus neuraminidase antibody 3A10|Homo sapiens (9606) SVLTQPPSVSGAPGQRVSISCTGSSSNIGAGFDVHWYQQVPGTAPKLLIYGNSNRPSGVPDRFSASKSGTSASLAITGLQPEDEADYYCQSYDNSLSVNYVFGTGTRVTVL |
Antigen
Chain: A
Mutation: NULL
| >8EZ3_A|Chain C[auth A]|Neuraminidase|Influenza A virus (A/Moscow/10/1999(H3N2)) (480019) AEYRNWSKPQCNITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPDKCYQFALGQGTTLNNGHSNDTVHDRTPYRTLLMNELGVPFHLGTKQVCIAWSSSSCHDGKAWLHVCVTGDDENATASFIYNGRLVDSIGSWSKKILRTQESECVCINGTCTVVMTDGSASGKADTKILFIEEGKIVHTSPLSGSAQHVEECSCYPRYPGVRCVCRDNWKGSNRPIVDINVKDYSIVSSYVCSGLVGDTPRKNDSSSSSHCLDPNNEEGGHGVKGWAFDDGNDVWMGRTISEKLRSGYETFKVIEGWSKPNSKLQINRQVIVDRGNRSGYSGIFSVEGKSCINRCFYVELIRGRKQETEVLWTSNSIVVFCGTSGTYGTGSWPDGADINLMPI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL32 ASP33 ASN52 SER53 ILE54 GLY55 ASN56 ALA57 PHE58 TYR59 GLN64 SER65 LEU67 THR68 ASN98 THR99 L: PHE31 TYR91 ASN93 SER94 A: SER88 LYS89 PRO90 GLN91 SER269 GLY270 SER271 ARG283 TYR284 PRO285 GLY286 ARG288 ASP304 ILE305 ASN306 LYS308 ASP309 SER311 ILE312 VAL313 SER314 SER315 CYS318 CYS337 LEU338 GLY357 ASN358 GLU381 TRP383 SER384 LYS385 PRO386 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
