Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6166 |
| PDBID: | 8EW6 |
| Chains: | V_W |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1007/s00259-022-05998-0 |
| Antibody | |
| Antibody: | Anti-human CD8 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human T-cell surface glycoprotein CD8 alpha chain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: V
Mutation: NULL
| >8EW6_V|Chain B[auth V]|Anti-CD8 alpha VHH|Lama glama (9844) APHHHHHHDDDDKEVQLVESGGGLVQAGGSLRLSCAASGFTFDDYAIGWFRQAPGKGREGVLCIRIFDRHTYSADSVKGRFTISSDNAQNTVYLHMNSLKPEDTAVYYCAAGSFWACTRPEGAMDYWGKGTQVTVSS |
Antigen
Chain: W
Mutation: NULL
| >8EW6_W|Chain A[auth W]|T-cell surface glycoprotein CD8 alpha chain|Homo sapiens (9606) SQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPGNSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| V: THR28 ASP30 ASP31 TYR32 ALA33 ARG52 PHE54 ASP55 HIS57 TYR59 GLY99 SER100 PHE101 TRP102 ALA103 THR105 ARG106 ALA110 ASP112 W: ARG4 LYS21 GLN23 VAL24 LEU25 LEU26 SER27 ASN28 PRO29 THR30 SER31 GLN54 ARG72 LEU73 GLY74 ASP75 THR76 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
