Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6150 |
| PDBID: | 8EPA |
| Chains: | AB_I |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1126/scitranslmed.abo0205 |
| Antibody | |
| Antibody: | REGN9432 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human interleukin receptor common gamma chain (IL2Rgamma) |
| Antigen mutation: | No |
| Durg Target: | P31785; P31785; |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8EPA_A|Chain D[auth A]|REGN9432 Fab heavy chain|Mus musculus (10090) EVQLVESGGGVVRPGGSLRLSCAASGFTFDDFDMSWVRQGPGKGLEWVSGINWHGSSTGYADSVKGRFTISRDNAKNSLYLQMSSLRAEDTALYHCVRGGTIVGATTPLDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVE |
Light Chain: B
Mutation: NULL
| >8EPA_B|Chain E[auth B]|REGN9432 Fab light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTMTCRASRTISSYLSWYQQKSGKVPNLLIFGASSLQSGVPSRFSASGSGTDFTLIISSLQPEDFATYYCQQSYSSPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >8EPA_I|Chain A[auth I]|Cytokine receptor common subunit gamma|Homo sapiens (9606) LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKENPFLFALEAEQKLISEEDLGGEQKLISEEDLHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: THR28 ASP30 ASP31 PHE32 ASN52 TRP53 HIS54 SER56 SER57 VAL103 GLY104 ALA105 THR106 THR107 PRO108 LEU109 B: SER31 TYR32 PHE49 GLY50 ALA51 SER91 TYR92 I: ARG168 THR169 ASP170 TRP171 ASP172 HIS173 TRP175 GLN194 ARG196 TYR197 THR198 ARG200 GLU217 TRP218 SER219 HIS220 PRO221 HIS223 TRP224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
