Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6149 |
| PDBID: | 8EPA |
| Chains: | HL_I |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1126/scitranslmed.abo0205 |
| Antibody | |
| Antibody: | REGN7257 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human interleukin receptor common gamma chain (IL2Rgamma) |
| Antigen mutation: | No |
| Durg Target: | P31785; P31785; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8EPA_H|Chain B[auth H]|REGN7257 Fab heavy chain|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGFIFSSYEMHWVRQAPGKGLEWISYISSSGTTIYYADSVKGRFTISRDNAKNSLYLHMNSLRAEDTAVYYCTRARITGTFDVFDIWGQGTMVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVE |
Light Chain: L
Mutation: NULL
| >8EPA_L|Chain C[auth L]|REGN7257 Fab light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIFAASNLQSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQNYNIPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >8EPA_I|Chain A[auth I]|Cytokine receptor common subunit gamma|Homo sapiens (9606) LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKENPFLFALEAEQKLISEEDLGGEQKLISEEDLHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ILE28 SER30 SER31 TYR32 GLU33 HIS35 TYR50 SER52 SER53 SER54 GLY55 THR56 ILE58 ASN74 ILE101 GLY103 THR104 ASP106 L: SER30 SER31 TYR32 ARG66 ASN91 TYR92 ASN93 ILE94 TYR96 I: LEU33 PRO34 GLU37 TRP68 LYS70 ASN71 SER72 ASP73 ASP75 LYS76 VAL77 GLN104 THR105 VAL107 GLN109 ARG117 ARG118 GLN119 ALA120 THR121 GLN122 MET123 LYS125 ASN128 LEU129 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
