Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 614 |
| PDBID: | 3P0Y |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.80 |
| Reference: | 10.1016/j.ccr.2011.09.003 |
| Antibody | |
| Antibody: | DL11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Epidermal growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | Q504U8; P00533; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3P0Y_H|Chain B[auth H]|Fab DL11 heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTLSGDWIHWVRQAPGKGLEWLGEISAAGGYTDYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARESRVSFEAAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >3P0Y_L|Chain C[auth L]|Fab DL11 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDLATDVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSEPEPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >3P0Y_A|Chain A|Epidermal growth factor receptor|Homo sapiens (9606) RKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKGNSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 GLY31 TRP33 GLU50 ALA53 GLY54 TYR56 THR57 ASP58 GLU95 SER96 ARG97 VAL98 SER99 L: THR31 ASP32 SER50 GLU92 PRO93 GLU94 TYR96 A: LEU382 GLN408 HIS409 GLY410 GLN411 PHE412 ALA415 VAL417 ASP436 ILE438 SER440 ASN449 TRP453 LYS454 PHE457 SER460 GLY461 GLN462 LYS463 THR464 LYS465 ILE466 ILE467 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
