Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6134 |
| PDBID: | 8EL2 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Severe acute respiratory syndrome coronavirus 2 |
| Method: | XRD |
| Resolution (Å): | 2.89 |
| Reference: | 10.1371/journal.ppat.1011532 |
| Antibody | |
| Antibody: | ICO-hu23 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8EL2_H|Chain A[auth H], C[auth I]|Fab ICO-hu23 Heavy Chain|Homo sapiens (9606) EVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYFHWVRQAPGQGLEWMGWINPISGGTKYAQKFQGWVTMTRATSISTVYMEVSRLRSDDTAVYYCARGHTYNWNYAYCDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHHHHHH |
Light Chain: L
Mutation: NULL
| >8EL2_L|Chain B[auth K], D[auth L]|Fab ICO-hu23 Light Chain|Homo sapiens (9606) SYVLTQPASVSGSPGQSITISCTGTSSDVGVYNYVSWYQQHPGKAPKLMIYDVSNRPSGVSNRFSGSKSGNTASLTISGLQAEDEADYYCSSYTSSSTLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >8EL2_A|Chain E[auth A], F[auth B]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) ASRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: TYR27 THR28 PHE29 THR30 GLY31 TYR33 TRP50 ASN52 ILE54 SER55 GLY57 THR58 LYS59 THR74 SER75 SER77 TYR102 ASN103 TRP104 ALA107 L: TYR32 TYR34 TYR93 THR94 SER95 SER97 A: LYS444 GLY446 TYR449 ASN450 LEU452 LEU455 PHE456 VAL483 GLU484 GLY485 PHE486 CYS488 TYR489 PHE490 LEU492 GLN493 SER494 TYR495 GLY496 GLN498 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
