Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 613 |
| PDBID: | 3P0G |
| Chains: | B_A |
| Organism: | Homo sapiens, Tequatrovirus T4, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/nature09648 |
| Antibody | |
| Antibody: | 3P0G Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-2 adrenergic receptor |
| Antigen mutation: | Yes |
| Durg Target: | P00720 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >3P0G_B|Chain B|Camelid Antibody Fragment|LAMA GLAMA (9844) QVQLQESGGGLVQAGGSLRLSCAASGSIFSINTMGWYRQAPGKQRELVAAIHSGGSTNYANSVKGRFTISRDNAANTVYLQMNSLKPEDTAVYYCNVKDYGAVLYEYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: N195E
| >3P0G_A|Chain A|Beta-2 adrenergic receptor, Lysozyme|Homo sapiens (9606) DYKDDDDAMGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYAEETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: SER27 ILE28 PHE29 SER30 ILE31 THR33 ALA50 HIS52 GLY54 SER56 THR57 ASN58 LYS98 TYR100 GLY101 ALA102 VAL103 LEU104 TYR105 GLU106 TYR107 A: THR66 THR68 ILE72 ILE127 ARG131 ALA134 ILE135 THR136 SER137 PRO138 PHE139 LYS140 GLN142 TYR219 VAL222 GLU225 ALA226 LEU266 LYS267 GLU268 ALA271 LEU272 THR274 LEU275 ILE278 ILE325 TYR326 ARG328 SER329 PRO330 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
