Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6120 |
| PDBID: | 8EEE |
| Chains: | AB_Z |
| Organism: | Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013, Macaca mulatta |
| Method: | XRD |
| Resolution (Å): | 2.82 |
| Reference: | 10.1016/j.celrep.2023.112942 |
| Antibody | |
| Antibody: | rhMZ104-D Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ZIKV virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8EEE_A|Chain D[auth A], E[auth H]|rhMZ104-D antibody heavy chain|Macaca mulatta (9544) EVQLVESGGGLAKPGGSLRLSCAASGFTFSDYYMDWVRQAPGKGLEWVSRISNGGGSTWYADSVKGRFTISRENAKNTLYLQMNSLRAEDTAVYYCARERYCSGGVCYAGTKYFDYWGQGVLVTVSSASTKGPSVFPLAPSSRSTSESTAALGCLVKDYFPEPVTVSWNSGSLTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYVCNVNHKPSNTKVDKRVEIK |
Light Chain: B
Mutation: NULL
| >8EEE_B|Chain C[auth B], F[auth L]|rhMZ104-D antibody light chain|Macaca mulatta (9544) QPVLTQPPSLSASPGASARLPCTLSSDLSVGSKNMYWYQQKPGSAPRLFLYYYSDSDKQLGPGVPNRVSGSKETSSNTAFLLISGLQPEDEADYYCQVYDSSANWVFGGGTRLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVEVAWKADGSAVNAGVETTKPSKQSNNKYAASSYLSLTSDQWKSHKSYSCQVTHEGSTVEKTVAPAE |
Antigen
Chain: Z
Mutation: NULL
| >8EEE_Z|Chain A[auth E], B[auth Z]|Envelope protein E|Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013 (2043570) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER103 GLY105 VAL106 CYS107 TYR108 ALA109 GLY110 LYS112 B: ASP27 LEU28 GLY31 SER32 LYS33 ASN34 TYR53 SER56 ASP57 TYR99 ASP100 SER101 SER102 Z: ASP67 ALA69 SER70 ASP71 ARG73 GLN77 GLU79 TYR81 LEU82 ASP83 LYS84 GLN85 SER86 ASP87 THR88 GLN89 TYR90 LYS118 THR233 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
