Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6118 |
| PDBID: | 8EED |
| Chains: | MN_D |
| Organism: | Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013, Macaca mulatta |
| Method: | XRD |
| Resolution (Å): | 3.49 |
| Reference: | 10.1016/j.celrep.2023.112942 |
| Antibody | |
| Antibody: | rhMZ107-B Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ZIKV virus envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >8EED_M|Chain E, F[auth H], H[auth I], K[auth M]|rhMZ107-B antibody heavy chain|Macaca mulatta (9544) QVQLQESGPGLVKPSETLSLTCAVSGYSISSGYYWGWIRQPPGKGLEYIGYISGSSGSTYYNPSLKSRVTISKDTSKNQFSLKLSSVTAADTAVYYCARRDRVGSYPYYYGLDSWGQGVLVTVSSASTKGPSVFPLAPSSRSTSESTAALGCLVKDYFPEPVTVSWNSGSLTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYVCNVNHKPSNTKVDKRVEIK |
Light Chain: N
Mutation: NULL
| >8EED_N|Chain G[auth F], I[auth J], J[auth L], L[auth N]|rhMZ107-B antibody light chain|Macaca mulatta (9544) QSVLTQPPSLSASPGASARLPCTLSSDLSVGSKNMYWYQQKPGSAPRLFLYYYSDSDKQLGPGVPNRVSGSKETSSNTAFLLISGLQPEDEADYYCQVYDGSANDVFGSGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVEVAWKADGSAVNAGVETTKPSKQSNNKYAASSYLSLTSDQWKSHKSYSCQVTHEGSTVEKTVAPAE |
Antigen
Chain: D
Mutation: NULL
| >8EED_D|Chain A, B, C, D|Envelope protein E|Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013 (2043570) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: TYR34 SER53 SER55 SER56 SER58 TYR60 ARG102 VAL103 GLY104 TYR106 PRO107 TYR110 N: SER32 ASN34 TYR51 TYR53 SER54 SER56 ASP57 LYS58 GLN59 GLY101 SER102 D: ILE65 SER66 ASP67 MET68 ALA69 SER70 ASP71 SER72 ARG73 CYS74 GLN77 ASP83 LYS84 VAL97 ARG99 TRP101 GLY102 ASN103 GLY104 CYS105 GLY106 LEU107 LEU113 LYS251 ARG252 GLN253 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
