Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6110 |
| PDBID: | 8EBP |
| Chains: | IM_B |
| Organism: | Human metapneumovirus A, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.38 |
| Reference: | 10.1016/j.chom.2023.07.002 |
| Antibody | |
| Antibody: | RSV-199 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human metapneumovirus (HMPV) A fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >8EBP_I|Chain C[auth H], F[auth I]|RSV-199 heavy chain protein|Homo sapiens (9606) QVQLVESGGGVVKPGGSLRVSCVVSGFTFSSYRMHWVRQAPGKGLEWVSSITASSSYINYAESVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDENTGISHYWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: M
Mutation: NULL
| >8EBP_M|Chain B[auth L], E[auth M]|RSV-199 light chain protein|Homo sapiens (9606) QAVVTQPPSVSGAPGQRVIISCTGSGSNLGADYGVHWYQQLPGTAPKLLIYGDRNRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDRSLNWVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVNAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPAECS |
Antigen
Chain: B
Mutation: NULL
| >8EBP_B|Chain A, D[auth B]|Fusion glycoprotein F0|Human metapneumovirus A (3037664) LKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRRRRFVLGAIALGVCTAAAVTAGVAIAKTIRLESEVTAIKNALKKTNEAVSTLGNGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPNEKDCETRGDHVFCDTACGINVAEQSKECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: ASP30 TYR31 TYR91 ARG93 ASN95 TRP96 I: THR28 SER30 SER31 TYR32 ARG33 THR52 ALA53 SER54 TYR56 ILE57 ASN58 ASN97 THR98 GLY99 B: LEU36 THR41 TRP43 TYR44 VAL148 ARG156 SER232 ASN233 MET234 PRO235 THR236 SER237 ALA238 GLY239 GLN240 ILE241 LYS242 LEU243 GLU246 ILE275 GLY277 ILE279 ASP280 THR281 PRO282 GLN312 ASN313 ALA314 GLY315 HIS332 GLU345 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
