Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6105 |
| PDBID: | 8EAY |
| Chains: | HL_A |
| Organism: | Human metapneumovirus A, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.33 |
| Reference: | 10.1016/j.chom.2023.07.002 |
| Antibody | |
| Antibody: | 4I3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human metapneumovirus (HMPV) A fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8EAY_H|Chain B[auth H]|4I3 heavy chain protein|Homo sapiens (9606) QVQLQESGPGLVKPSETLSLTCTVSGGSISNYYWNWIRQPPGKGLEWIGYFYYSGSTKYNPSLKSRVTISLDMSKNQFSLKLRSVTAADTAVYYCARGTMRESGMPDAFDIWGQGTVVTVS |
Light Chain: L
Mutation: NULL
| >8EAY_L|Chain C[auth L]|4I3 light chain protein|Homo sapiens (9606) SYELIQPPSVSVSPGQTAIITCSGNNLGNKYACWYQQKAGQSPVMIIYQDNRRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCQAWDSSVVFGGGTKLTVLGQ |
Antigen
Chain: A
Mutation: NULL
| >8EAY_A|Chain A|Fusion glycoprotein F0|Human metapneumovirus A (3037664) LKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRQSRFVLGAIALGVCTAAAVTAGVAIAKTIRLESEVTAIKNALKKTNEAVSTLGNGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPNEKDCETRGDHVFCDTACGINVAEQSKECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 ASN31 TYR33 TYR50 TYR52 TYR53 SER54 GLY55 SER56 THR57 LYS58 MET97 ARG98 GLU99 L: ASN26 ASN27 LEU28 GLY29 ASN30 LYS31 TYR32 GLN50 ASP51 ASN66 GLY68 ASN69 TRP91 SER93 A: LYS20 GLU21 SER22 TYR23 LEU24 GLU25 GLU26 ILE31 GLU33 GLY34 TRP284 GLN312 GLU345 LYS348 GLU349 ASN351 ILE352 ILE354 THR356 THR357 ASN358 LEU378 TYR409 THR411 GLN413 ASP414 GLU431 GLY432 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
