Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6103 |
| PDBID: | 8E8Z |
| Chains: | HL_1 |
| Organism: | Human poliovirus 1 strain Sabin, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.15 |
| Reference: | 10.1038/s41467-023-41052-9 |
| Antibody | |
| Antibody: | 9H2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human poliovirus 1 strain Sabin capsid protein VP1 |
| Antigen mutation: | No |
| Durg Target: | P03300 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8E8Z_H|Chain E[auth H]|9H2 Fab heavy chain|Homo sapiens (9606) LVQSGAELKKPGASVKFSCQASGFTFTTYDIHWVRQAPGQGLEWMGMISPSRDSTIYAQKFQGRVTMTSDTSTSTVYMELTSLRSEDTALYYCATASRPSAWVFRSLYTYYYMDVWGTGTTVTV |
Light Chain: L
Mutation: NULL
| >8E8Z_L|Chain F[auth L]|9H2 Fab light chain|Homo sapiens (9606) SALTQPASVSGSPGQSITISCTGTITDIGYYNYVSWYQQHPGKAPKLIIFDVTNRPSGVSDRFSGSKSGNTASLTISGLQAEDEGDYYCFSHRSNNIRVFGGGTKLTVL |
Antigen
Chain: 1
Mutation: NULL
| >8E8Z_1|Chain A[auth 1]|Capsid protein VP1|Human poliovirus 1 strain Sabin (12082) TSRDALPNTEASGPAHSKEIPALTAVETGATNPLVPSDTVQTRHVVQHRSRSESSIESFFARGACVAIITVDNSASTKNKDKLFTVWKITYKDTVQLRRKLEFFTYSRFDMEFTFVVTANFTETNNGHALNQVYQIMYVPPGAPVPEKWDDYTWQTSSNPSIFYTYGTAPARISVPYVGISNAYSHFYDGFSKVPLKDQSAALGDSLYGAASLNDFGILAVRVVNDHNPTKVTSKIRVYLKPKHIRVWCPRPPRAVAYYGPGVDYKDGTLTPLSTKDLTTY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR51 SER119 ARG120 PRO121 SER122 ALA123 TRP124 VAL125 ARG127 SER128 LEU129 THR131 TYR133 ASP136 L: THR42 GLY43 THR44 ILE45 THR46 GLY49 TYR50 TYR51 ASN52 PHE70 THR73 ASN74 ARG75 PRO76 SER77 ASN90 ASN115 1: CYS86 VAL87 ALA88 ILE89 ILE90 THR91 ASN100 LYS101 ASP102 LEU104 PHE105 THR106 VAL107 TRP108 LYS109 ASP114 THR115 VAL166 PRO167 GLU168 LYS169 LYS214 ALA223 LEU224 ASP226 SER227 LEU228 ALA231 LEU234 ILE239 LYS252 TYR280 GLY281 PRO282 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
