Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6101 |
| PDBID: | 8E8X |
| Chains: | HL_1 |
| Organism: | Human poliovirus 3 strain Sabin, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.91 |
| Reference: | 10.1038/s41467-023-41052-9 |
| Antibody | |
| Antibody: | 9H2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human poliovirus 3 strain Sabin capsid protein VP1 |
| Antigen mutation: | No |
| Durg Target: | P03302 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8E8X_H|Chain E[auth H]|9H2 Fab heavy chain|Homo sapiens (9606) LVQSGAELKKPGASVKFSCQASGFTFTTYDIHWVRQAPGQGLEWMGMISPSRDSTIYAQKFQGRVTMTSDTSTSTVYMELTSLRSEDTALYYCATASRPSAWVFRSLYTYYYMDVWGTGTTVTVSS |
Light Chain: L
Mutation: NULL
| >8E8X_L|Chain F[auth L]|9H2 Fab light chain|Homo sapiens (9606) QSALTQPASVSGSPGQSITISCTGTITDIGYYNYVSWYQQHPGKAPKLIIFDVTNRPSGVSDRFSGSKSGNTASLTISGLQAEDEGDYYCFSHRSNNIRVFGGGTKLTVL |
Antigen
Chain: 1
Mutation: NULL
| >8E8X_1|Chain A[auth 1]|Capsid protein VP1|Human poliovirus 3 strain Sabin (270338) QDSLPDTKASGPAHSKEVPALTAVETGATNPLAPSDTVQTRHVVQRRSRSESTIESFFARGACVAIIEVDNEQPTTRAQKLFAMWRITYKDTVQLRRKLEFFTYSRFDMEFTFVVTANFTNANNGHALNQVYQIMYIPPGAPTPKSWDDYTWQTSSNPSIFYTYGAAPARISVPYVGLANAYSHFYDGFAKVPLKTDANDQIGDSLYSAMTVDDFGVLAVRVVNDHNPTKVTSKVRIYMKPKHVRVWCPRPPRAVPYYGPGVDYRNNLDPLSEKGLTTY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER44 PHE46 THR47 TYR51 GLN84 SER119 ARG120 PRO121 SER122 ALA123 TRP124 VAL125 ARG127 SER128 LEU129 THR131 TYR133 ASP136 L: THR42 GLY43 THR44 ILE45 THR46 GLY49 TYR50 TYR51 ASN52 PHE70 THR73 ASN74 ASN90 ASN115 1: CYS86 VAL87 ALA88 ILE89 ILE90 GLU91 ARG100 ALA101 GLN102 LYS103 PHE105 ALA106 MET107 TRP108 ARG109 ASP114 THR115 VAL116 THR166 LYS168 LYS214 GLN224 ASP227 SER228 LEU229 VAL235 ASP236 VAL240 TYR281 GLY282 PRO283 ARG288 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
