Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6100 |
| PDBID: | 8E8S |
| Chains: | HL_1 |
| Organism: | Poliovirus 2, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.73 |
| Reference: | 10.1038/s41467-023-41052-9 |
| Antibody | |
| Antibody: | 9H2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human poliovirus 2 strain WT capsid protein VP1 |
| Antigen mutation: | No |
| Durg Target: | P06210 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8E8S_H|Chain E[auth H]|9H2 Fab heavy chain|Homo sapiens (9606) LVQSGAELKKPGASVKFSCQASGFTFTTYDIHWVRQAPGQGLEWMGMISPSRDSTIYAQKFQGRVTMTSDTSTSTVYMELTSLRSEDTALYYCATASRPSAWVFRSLYTYYYMDVWGTGTTVTVSS |
Light Chain: L
Mutation: NULL
| >8E8S_L|Chain F[auth L]|9H2 Fab light chain|Homo sapiens (9606) QSALTQPASVSGSPGQSITISCTGTITDIGYYNYVSWYQQHPGKAPKLIIFDVTNRPSGVSDRFSGSKSGNTASLTISGLQAEDEGDYYCFSHRSNNIRVFGGGTKLTVL |
Antigen
Chain: 1
Mutation: NULL
| >8E8S_1|Chain A[auth 1]|Capsid protein VP1|Poliovirus 2 (12083) NNLPDTQSSGPAHSKETPALTAVETGATNPLVPSDTVQTRHVIQKRTRSESTVESFFARGACVAIIEVDNDAPTKRASKLFSVWKITYKDTVQLRRKLEFFTYSRFDMEFTFVVTSNYTDANNGHALNQVYQIMYIPPGAPIPGKWNDYTWQTSSNPSVFYTYGAPPARISVPYVGIANAYSHFYDGFAKVPLAGQASTEGDSLYGAASLNDFGSLAVRVVNDHNPTKLTSKIRVYMKPKHVRVWCPRPPRAVPYYGPGVDYKDGLAPLPGKGLTTY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: PHE46 TYR51 SER119 ARG120 PRO121 SER122 ALA123 TRP124 VAL125 ARG127 SER128 LEU129 THR131 TYR133 L: GLN20 THR42 GLY43 THR44 ILE45 THR46 GLY49 TYR50 ASN52 THR73 ASN74 ARG75 ASN90 ASN115 1: CYS86 VAL87 ALA88 ILE89 ILE90 GLU91 SER102 LYS103 PHE105 SER106 VAL107 TRP108 LYS109 ASP114 THR115 VAL116 ILE166 GLY168 LYS169 LYS214 THR223 ASP226 SER227 LEU228 LEU234 ASN235 SER239 LYS252 TYR279 TYR280 GLY281 PRO282 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
