Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6096 |
| PDBID: | 8E6K |
| Chains: | GK_D |
| Organism: | Influenza A virus (A/Brevig Mission/1/1918(H1N1)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1016/j.immuni.2023.07.004 |
| Antibody | |
| Antibody: | 2H08 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus neuraminidase from Brevig Mission/1/1918 H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >8E6K_G|Chain C[auth E], F, I[auth G], L[auth H]|2H08 fragment antigen binding heavy chain|Homo sapiens (9606) QVQLQESGPGLVKPSETLSLTCTVSGDSVSSTNYYWGWIRQPPGKGLEWIGSIYYRGITYNSPSLMNRVTISLDTAKNQFSLNLSSMTAADTAVYFCANSIAVSGPLYFHHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: K
Mutation: NULL
| >8E6K_K|Chain B[auth I], E[auth J], H[auth K], K[auth L]|2H08 fragment antigen binding light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSLSGYLNWYQQKPGKAPKLLIYATSTLQRGVPSRFSGSGSGTDFTLTISSLQPEDFAIYYCQQSYSTPPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >8E6K_D|Chain A, D[auth B], G[auth C], J[auth D]|Neuraminidase|Influenza A virus (A/Brevig Mission/1/1918(H1N1)) (88776) ADPHHHHHHSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSLVPRGSPSRSVILTGNSSLCPISGWAIYSKDNGIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPYRTLMSCPVGEAPSPYNSRFESVAWSASACHDGMGWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTIMTDGPSNGQASYKILKIEKGKVTKSIELNAPNYHYEECSCYPDTGKVMCVCRDNWHGSNRPWVSFDQNLDYQIGYICSGVFGDNPRPNDGTGSCGPVSSNGANGIKGFSFRYDNGVWIGRTKSTSSRSGFEMIWDPNGWTETDSSFSVRQDIVAITDWSGYSGSFVQHPELTGLDCMRPCFWVELIRGQPKENTIWTSGSSISFCGVNSDTVGWSWPDGAELPFSIDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: SER28 SER30 GLY31 TYR32 TYR49 ALA50 THR53 GLY66 SER67 GLY68 TYR92 SER93 THR94 G: THR32 ASN33 TYR52 ARG54 ILE56 TYR58 ALA97 VAL98 SER99 D: ASN88 SER89 SER90 LEU91 PRO93 ILE94 SER95 TYR356 ASP357 ASN358 TRP361 TRP378 PRO380 ASN381 THR384 GLU385 THR386 ASP387 SER389 PHE390 SER391 GLY414 ASP416 CYS417 ASN450 SER451 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
