Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6090 |
| PDBID: | 8E6J |
| Chains: | EI_B |
| Organism: | Influenza A virus (A/Brevig Mission/1/1918(H1N1)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.immuni.2023.07.004 |
| Antibody | |
| Antibody: | 3H03 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus neuraminidase from Brevig Mission/1/1918 H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >8E6J_E|Chain C[auth E], F, I[auth G], L[auth H]|3H03 fragment antigen binding heavy chain|Homo sapiens (9606) QVQLQESGPGLVKPSETLSLTCTVSGDSISSSYYYWGWIRQSPVKGLEWIGSFFYSGNTNYNPSLKSRVTISVDTSKNQFSLNLRSVTAADTAVYYCARHVTSISSWNRGVYLDSWGRGALVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: I
Mutation: NULL
| >8E6J_I|Chain B[auth I], E[auth J], H[auth K], K[auth L]|3H03 fragment antigen binding light chain|Homo sapiens (9606) DIQMTQSPSSLSTSVGDRVTITCRASQTISTYLNWYQQKPGKAPELLIYVASSLQSGVPSRFSGTGSGTEFTLTISSLQPGDFATYYCQQSYSSPFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >8E6J_B|Chain A, D[auth B], G[auth C], J[auth D]|Neuraminidase|Influenza A virus (A/Brevig Mission/1/1918(H1N1)) (88776) ADPHHHHHHSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSLVPRGSPSRSVILTGNSSLCPISGWAIYSKDNGIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPYRTLMSCPVGEAPSPYNSRFESVAWSASACHDGMGWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTIMTDGPSNGQASYKILKIEKGKVTKSIELNAPNYHYEECSCYPDTGKVMCVCRDNWHGSNRPWVSFDQNLDYQIGYICSGVFGDNPRPNDGTGSCGPVSSNGANGIKGFSFRYDNGVWIGRTKSTSSRSGFEMIWDPNGWTETDSSFSVRQDIVAITDWSGYSGSFVQHPELTGLDCMRPCFWVELIRGQPKENTIWTSGSSISFCGVNSDTVGWSWPDGAELPFSIDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASP27 SER28 ILE29 SER30 SER31 SER32 TYR33 TYR53 SER54 ASN56 VAL71 THR73 SER74 THR97 SER98 ILE99 B: SER90 LEU91 CYS92 PRO93 ILE94 SER95 ASP283 THR284 ARG355 TYR356 ASP357 ASN358 GLY359 TRP361 TRP378 PRO380 ASN381 GLU385 VAL392 LEU415 ASP416 CYS417 ARG419 VAL449 ASN450 SER451 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
