Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6085 |
| PDBID: | 8E0E |
| Chains: | B_A |
| Organism: | Rattus norvegicus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1038/s41467-022-35025-7 |
| Antibody | |
| Antibody: | F3 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rattus norvegicus Voltage-dependent L-type calcium channel subunit beta-2 |
| Antigen mutation: | No |
| Durg Target: | Q08289; Q08289; |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >8E0E_B|Chain B|Nanobody nb.F3|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCVASGRTFSSYAMGWFRQAPGKEREFVAAIDWSGGTASHADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAASSYWSRSVDEYDYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >8E0E_A|Chain A|Voltage-dependent L-type calcium channel subunit beta-2|Rattus norvegicus (10116) GHMGSADSYTSRPSDSDVSLEEDREAVRREAERQAQAQLEKAKTKPVAFAVRTNVRYSAAQEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGCEIGFIPSPVKLENMRLQHEQRAKQGSSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADISLAKRSVLNNPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTINHPAQLSKTSLAPIIVYVKISSPKVLQRLIKSRGKSQAKHLNVQMVAADKLAQCPPQESFDVILDENQLEDACEHLADYLEAYWKATHPPSSNLPNPLLSRT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: SER18 ARG20 ARG28 THR29 SER31 SER32 TYR33 ILE52 ASP53 TRP54 SER55 GLY56 GLY57 THR58 ALA59 SER60 HIS61 LYS66 GLY67 ARG68 THR70 ILE71 SER72 ARG73 ASP74 ASN75 TYR81 GLN83 ASN85 SER86 SER101 TYR102 TRP103 GLU109 ASP111 TYR112 A: ALA61 PHE62 VAL95 GLU97 LYS98 ASN101 TRP104 SER120 PRO121 VAL122 GLU125 ARG128 HIS216 PRO218 PRO219 TYR220 ASP221 VAL222 ALA273 LYS274 ARG275 LEU278 ASN279 PRO281 LYS283 ILE286 ARG289 SER290 THR292 ALA297 GLN300 SER301 ILE303 GLU304 PHE307 SER333 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
