Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6078 |
| PDBID: | 8DYP |
| Chains: | B_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1016/j.cell.2022.08.020 |
| Antibody | |
| Antibody: | P10 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cystine transporter cystinosin |
| Antigen mutation: | Yes |
| Durg Target: | O60931 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >8DYP_B|Chain B|Nanobody P10|synthetic construct (32630) ESGGGLVQAGGSLRLSCAASGSISPKNWMGWYRQAPGKEREFVATIDYGANTNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAADYDYGDRQRYGHLYWGQGTQVTVSSH |
Antigen
Chain: A
Mutation: T236I
| >8DYP_A|Chain A|Cystinosin|Homo sapiens (9606) SLTVPPVVKLEAGSSTAVSLTLRPPLAATLVITFEITFRSKAITILELPDEVVVPPGVTASSFQVTSQNVGQLTVYLHGAHSAQTGPRIRFLVIRSSAISIINQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSNDVFFSLHAVVLTLIIIVQCCLYERGGQRVSWPAIGFLVLAWLFAFVTMIVAAVGVITWLQFLFCFSYIKLAVTLVKYFPQAYMNFYYKSTEGWSIGAVLLDFTGGSFSLLQMFLQSYNNDQWTLIFGDPTKFGLGVFSIVFDVVFFIQHFCL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: LYS33 TRP35 TYR39 PHE49 THR52 ASP54 TYR55 ASN58 ASN60 TYR61 ASP100 A: THR53 PRO73 ASP74 GLU75 VAL76 VAL77 PRO79 PRO80 GLY81 VAL82 ALA84 SER85 SER86 PHE87 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
