Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 606 |
| PDBID: | 3NPS |
| Chains: | BC_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.50 |
| Reference: | 10.1016/j.jmb.2011.11.036 |
| Antibody | |
| Antibody: | S4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Suppressor of tumorigenicity 14 protein |
| Antigen mutation: | Yes |
| Durg Target: | Q9Y5Y6 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >3NPS_B|Chain B|S4 FAB HEAVY CHAIN|Homo sapiens (9606) EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAGQGLEWMGGIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARTFHIRRYRSGYYDKMDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLESSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: C
Mutation: NULL
| >3NPS_C|Chain C|S4 FAB LIGHT CHAIN|Homo sapiens (9606) VLTQPPSVSGAPGQRVTISCSGSSSNIGSNYVSWYQQKPGTAPKLLIYDNNQRPSGVPDRFSGSKSGTSAVLAITGLQSEDEADYYCQSRDISQYVFGGGTKLTVLRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG |
Antigen
Chain: A
Mutation: C117S
| >3NPS_A|Chain A|Suppressor of tumorigenicity 14 protein|Homo sapiens (9606) VVGGTDADEGEWPWQVSLHALGQGHICGASLISPNWLVSAAHCYIDDRGFRYSDPTQWTAFLGLHDQSQRSAPGVQERRLKRIISHPFFNDFTFDYDIALLELEKPAEYSSMVRPISLPDASHVFPAGKAIWVTGWGHTQYGGTGALILQKGEIRVINQTTCENLLPQQITPRMMCVGFLSGGVDSCQGDSGGPLSSVEADGRIFQAGVVSWGDGCAQRNKPGVYTRLPLFRDWIKENTGV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLU1 SER30 SER31 TYR32 ILE53 PHE54 HIS97 ILE98 ARG99 ASP101 HIS102 C: GLY29 SER30 ASN31 TYR32 ASP50 ASN51 ASN52 GLN53 PRO55 SER56 GLY57 LYS66 ARG91 A: ILE41 CYS42 HIS57 CYS58 ASP96 PHE97 THR98 PHE99 HIS143 TYR146 GLY147 GLY148 THR150 PRO173 GLN174 GLN175 ASP189 SER190 CYS191 GLN192 SER195 VAL213 TRP215 GLY216 GLY219 CYS220 GLN221 GLY226 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
