Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6058 |
| PDBID: | 8DWO |
| Chains: | HL_B |
| Organism: | Eastern equine encephalitis virus, Macaca fascicularis |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1016/j.cell.2023.05.019 |
| Antibody | |
| Antibody: | SKE26 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Eastern equine encephalitis virus spike glycoprotein E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8DWO_H|Chain E[auth H], I[auth S], K[auth U]|SKE26 Fab Heavy Chain|Macaca fascicularis (9541) VVQLVQSGAEVKRPGESLRISCKTSGYRFTDIWINWVRQRPGKGLEWMGSIYLDDSDIRFNPSFQGHVTISADKSTSTTYLQWSSLKASDTATYFCARSVWSGYYIGWFDVWGPGVLVTVSSASTKGPSVFPLAPSSRSTSESTAALGCLVKDYFPEPVTVSWNSGSLTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYVCNVNHKPSNTKVDKRVEIKTCGGLEVLFQ |
Light Chain: L
Mutation: NULL
| >8DWO_L|Chain H[auth L], J[auth T], L[auth V]|SKE26 Fab Light Chain|Macaca fascicularis (9541) DVVMTQTPLSLPVTPGEPASISCRSSQSLLDSNGNTYLHWYLQKPGQSPQLLIYGGSNRASGVPDRFSGSGSGTDFTLTINNVEAGDVGTYYCMQVIQVPLTFGGGTKVDIKRTVAAPSVFIFPPSEDQVKSGTVSVVCLLNNFYPREASVKWKVDGALKTGNSQESVTEQDSKDNTYSLSSTLTLSSTEYQSHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >8DWO_B|Chain B, D[auth G], G[auth K]|Envelope glycoprotein E2|Eastern equine encephalitis virus (11021) DLDTHFTQYKLARPYIADCPNCGHSRCDSPIAIEEVRGDAHAGVIRIQTSAMFGLKTDGVDLAYMSFMNGKTQKSIKIDNLHVRTSAPCSLVSHHGYYILAQCPPGDTVTVGFHDGPNRHTCTVAHKVEFRPVGREKYRHPPEHGVELPCNRYTHKRADQGHYVEMHQPGLVADHSLLSIHSAKVKITVPSGAQVKYYCKCPDVRKGITSSDHTTTCTDVKQCRAYLIDNKKWVYNSGRLPRGEGDTFKGKLHVPFVPVKAKCIATLAPEPLVEHKHRTLILHLHPDHPTLLTTRSLGSDANPTRQWIERPTTVNFTVTGEGLEYTWGNHPPKRVWAQESGEGNPHGWPHEVVVYYYNRYPLTTIIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYKLAPNAQVPILLALLCCIKPTRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 ARG28 PHE29 ASP31 TRP33 ASP53 ASP54 ASP56 ARG58 ARG94 VAL96 TRP97 SER98 GLY99 ASP101 L: SER27 ASN28 ASN30 TYR32 TYR49 VAL91 ILE92 GLN93 VAL94 LEU96 B: ASP3 PHE6 TYR9 LYS10 LEU11 ARG13 THR57 ASP58 GLY59 MET68 ASN69 GLY70 LYS71 THR72 GLN73 LYS74 SER75 VAL172 SER176 LYS186 THR188 VAL189 PRO190 SER191 GLY192 ALA193 GLN194 THR209 SER210 SER211 LEU227 ILE228 ASP229 ASN230 LYS231 LYS232 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
