Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6038 |
| PDBID: | 8DTO |
| Chains: | GH_E |
| Organism: | Human immunodeficiency virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.57 |
| Reference: | 10.1016/j.chom.2024.04.006 |
| Antibody | |
| Antibody: | MU89 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 10.17DT SOSIP envelope glycoprotein - gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >8DTO_G|Chain C, G, K|MU89 Heavy Chain|Mus musculus (10090) QVQLVQSGAEVKKPGASVKVSCKASGYRFTDHYIHWVRQAPGQGPEWMGWINTSSGRSNFAQKFQGRVTMTRDTSISTAYMELNRLKSDDTAVYYCTTGSWISLYYDSSGYPNFDYWGQGTLVTVT |
Light Chain: H
Mutation: NULL
| >8DTO_H|Chain D, H, L|MU89 Light Chain|Mus musculus (10090) TQPASVSGSPGQPITISCTGTSSDVGNYDLVSWYQQHPGNAPKYMIYEVTKRPAGISNRFSGSKSGNTASLTISGLQAEDAADYYCCSYAGSSTVIFGGGTKVTVL |
Antigen
Chain: E
Mutation: NULL
| >8DTO_E|Chain A, E, I|CH848.3.D0949.10.17chim.6R.DS.SOSIP.664_N133D_N138T gp120|Human immunodeficiency virus 1 (12721) MGSLQPLATLYLLGMLVASVLAAENLWVTVYYGVPVWKEAKTTLFCASDARAYEKEVHNVWATHACVPTDPSPQELVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLICSDATVKTGTVEEMKNCSFNTTTEIRDKEKKEYALFYKPDIVPLSETNNTSEYRLINCNTSACTQACPKVTFEPIPIHYCAPAGYAILKCNDETFNGTGPCSNVSTVQCTHGIRPVVSTQLLLNGSLAEKEIVIRSENLTNNAKIIIVHLHTPVEIVCTRPNNNTRKSVRIGPGQTFYATGDIIGDIKQAHCNISEEKWNDTLQKVGIELQKHFPNKTIKYNQSAGGDMEITTHSFNCGGEFFYCNTSNLFNGTYNGTYISTNSSANSTSTITLQCRIKQIINMWQGVGRCMYAPPIAGNITCRSNITGLLLTRDGGTNSNETETFRPAGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: TYR33 TRP50 ASN52 SER54 SER55 GLY56 ARG57 SER58 ASN59 ARG72 ILE102 SER103 LEU104 TYR105 TYR106 ASP107 H: SER96 SER97 E: ALA134 THR135 VAL136 LYS137 THR138 THR148 VAL149 PRO299 ASN300 ASN301 ILE322 ILE323 GLY324 ASP325 ILE326 LYS327 GLN328 HIS330 THR414 GLN416 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
