Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6033 |
| PDBID: | 8DS5 |
| Chains: | CB_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.93 |
| Reference: | 10.1136/jitc-2022-005049 |
| Antibody | |
| Antibody: | MK5890 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Boserolimab(Phase-II) |
| Antigen | |
| Antigen: | Human CD27 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >8DS5_C|Chain C|MK-5890 Fab heavy chain|Mus musculus (10090) EIQLVQSGAEVKKPGASVKVSCKASGYTFTNYGMNWVKQAPGQGLKWMGWINTNTGEPTYAEEFKGRFTFTLDTSISTAYMELSSLRSEDTAVYYCAREGDAMDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: B
Mutation: NULL
| >8DS5_B|Chain B|MK-5890 Fab light chain|Mus musculus (10090) EIVLTQSPATLSLSPGERATLSCSASSSVSYMHWYQQKPGQAPKRWIYDTSKLASGVPARFSGSGSGTDYSLTISSLEPEDFAVYYCQQWNSYPFTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >8DS5_A|Chain A|CD27 antigen|Homo sapiens (9606) TPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR31 HIS33 ASP49 LYS52 TRP90 TYR93 PHE95 C: THR30 ASN31 TYR32 GLY33 TRP50 ASN52 THR53 ASN54 THR55 GLU99 GLY100 ASP101 ALA102 A: PRO28 GLU29 HIS31 LYS37 LEU38 GLN41 LYS51 ASP54 GLN55 HIS56 ARG57 LYS58 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
