Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6025 |
| PDBID: | 8DPM |
| Chains: | DE_A |
| Organism: | Ebola virus - Mayinga, Zaire, 1976, Mus musculus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.00 |
| Reference: | 10.1016/j.celrep.2023.113366 |
| Antibody | |
| Antibody: | 9.20.1A2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus Mayinga/Zaire/1976 glycoprotein GP1 |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >8DPM_D|Chain D, I, N|Antibody 9.20.1A2 Fab heavy chain|Homo sapiens (9606) EVQLVESGGDLVQPGGSLRLSCAASGITLSGVWMNWVRQAPGKGLEWIGRIKSTSDGGRADFAAPARGRFTMSRDESKNKLFLQMNNLGIEDTGMYYCFTRVQRDGTKDDFWGRGTLVTVSS |
Light Chain: E
Mutation: NULL
| >8DPM_E|Chain E, J, O|Antibody 9.20.1A2 Fab light chain|Homo sapiens (9606) QSVLTQPPSVSGAPGQTVTISCTGSYSNIGAGYDVQWYQHLPGTAPKLLIYDNVHRPSGVPDRFSGSKSGTSASLAITGLQTEDEADYYCQSYDSRLRDQWVFGGGTKLTVL |
Antigen
Chain: A
Mutation: NULL
| >8DPM_A|Chain A, F, K|Glycoprotein GP1|Ebola virus - Mayinga, Zaire, 1976 (128952) IPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TRP33 ASN35 ARG50 LYS52 SER53 SER55 ASP56 GLY57 ARG59 ASP61 ARG101 GLN103 LYS108 E: TYR26 ALA31 GLY32 TYR33 ASP34 GLN36 ASP52 TYR93 SER95 ARG96 LEU97 ARG98 ASP99 TRP101 A: GLU112 LYS114 LYS115 PRO116 ASP117 GLY118 SER119 GLU120 SER142 GLY143 THR144 GLY145 PRO146 CYS147 ALA148 GLN221 THR223 GLY224 THR227 ASN228 GLU229 GLU231 LEU233 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
