Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6014 |
| PDBID: | 8DMH |
| Chains: | FE_A |
| Organism: | Mammarenavirus choriomeningitidis, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.19 |
| Reference: | 10.1016/j.chembiol.2023.03.005 |
| Antibody | |
| Antibody: | 18.5C-M28 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mammarenavirus choriomeningitidis glycoprotein G1 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >8DMH_F|Chain C[auth F], G[auth H], K[auth J]|18.5C-M28 Fab Heavy Chain|Homo sapiens (9606) DEVQLVQSGGGLVRPGGSLRLSCAAAGFTFKRYSMNWVRQAPGRGLEWVSSITSRGGSKTYYADVVKGRFTVSRDNAKQSLYLQMNSLRAEDTAIYFCARSLHSTSQPSYMDVWGRKITVIVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPK |
Light Chain: E
Mutation: NULL
| >8DMH_E|Chain B[auth E], F[auth G], J[auth I]|18.5C-M28 Fab Light Chain|Homo sapiens (9606) DIVLTQSPGTLSLSPGERATLSCRASQSVISYYVAWYQHKGGQAPRLLIYGASSRATGVPDRFSGSGSGTDFTLTISSLEPEDFALYYCQYYGSSPLWAFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG |
Antigen
Chain: A
Mutation: G167C/F220L/L224R/A225R
| >8DMH_A|Chain A, E[auth B], I[auth C]|Glycoprotein G1|Lymphocytic choriomeningitis virus (3052303) MKLCILLAVVAFVGLSLGMYGLKGPDIYKGVYQFKSVEFDMSHLNLTMPNACSANNSHHYISMGTSGLELTFTNDSIISHNFCNLTSAFNKKTFDHTLMSIVSSLHLSIRGNSNYKAVSCDFNNGITIQYNLTFSDAQSAQSQCRTFRGRVLDMFRTAFGGKYMRSCWGWTGSDGKTTWCSQTSYQYLIIQNRTWENHCTYAGPFGMSRILLSQEKTKFLTRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: SER31 GLY51 SER53 SER54 ARG55 ASP61 SER64 GLY65 SER66 GLY67 SER68 THR75 F: ARG55 GLY56 SER58 LYS59 TYR61 A: ASP66 ILE67 TYR68 LYS69 TRP208 GLY209 TRP210 GLY212 SER213 ASP214 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
