Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6000 |
| PDBID: | 8DL6 |
| Chains: | DC_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.00 |
| Reference: | 10.7554/eLife.82947 |
| Antibody | |
| Antibody: | 11F9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human solute carrier family 40 member 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >8DL6_D|Chain C[auth D]|11F9 heavy-chain|Mus musculus (10090) MKCSWVIFFLMAVVTGVNSEVQLQQSGAELVRPGALVKLSCKASGFNIKDYYMHWVKERPEQGLEWIGWIDPENGNTIYDPKFQGKASITADTSSNTAYLQLSSLTSEDTAVYYCARKRGYYGPYFDYWGQGTTLTVSSKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSGLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPA |
Light Chain: C
Mutation: NULL
| >8DL6_C|Chain B[auth C]|11F9 light-chain|Mus musculus (10090) DIVMTQSQKFMSTSVGDRVSITCKASQNVGTAVAWYQKKPGQSPKLLIYSASNRYSGVPDRFTGSGSGTDFTLTISNMQSEDLADYFCQQYGSYPLTFGSGTKLEIKEAEAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >8DL6_A|Chain A|Solute carrier family 40 member 1|Homo sapiens (9606) MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVVENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASN48 VAL49 GLY50 THR51 ALA52 TYR69 SER70 ASN73 SER87 TYR111 GLY112 SER113 TYR114 D: PHE46 LYS49 ASP50 TYR51 TYR52 TRP69 ASP71 GLU73 ASN74 GLY75 ASN76 THR77 ILE78 TYR79 GLN84 ARG119 GLY120 TYR121 TYR122 PRO124 TYR125 A: VAL160 GLY164 GLU165 ASP166 ARG167 SER168 LEU170 ALA237 TYR303 ASN304 PRO306 VAL307 ARG364 ARG365 LYS366 CYS367 GLY368 LEU369 VAL370 ARG371 LEU479 GLU482 ASN483 ILE485 SER487 ARG489 PHE541 ASN544 THR545 LEU546 GLY547 ASN548 LYS549 CYS553 PRO555 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
