Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5990 |
| PDBID: | 8DG8 |
| Chains: | HL_B |
| Organism: | Human parainfluenza 3 virus (strain NIH 47885), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.62 |
| Reference: | 10.1038/s41467-023-36459-3 |
| Antibody | |
| Antibody: | 3x1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human paraInfluenza 3 virus (HPIV3) NIH 47885 fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8DG8_H|Chain B[auth H]|3x1 monoclonal antibody, VH region|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSFGMSWVRQSPGKGLEWVADISHSAGFLNYADSVKGRFTVSRDNSKSTLHLQMKSLRAEDTAVYYCAKRLAGLPDLEWLLYPNFLDHWGQGTLVTVSS |
Light Chain: L
Mutation: NULL
| >8DG8_L|Chain C[auth L]|3x1 monoclonal antibody, VL region|Homo sapiens (9606) SSELTQDPAVSVALGQTVRITCQGDILRTYYVSWYQQKPGQAPLLVIYGKNNRPSVIPDRFSGSTSGDTASLTITGAQAEDEAEYYCSSRDRSGNHVLFGGGTKLTVL |
Antigen
Chain: B
Mutation: NULL
| >8DG8_B|Chain A, D[auth B], E[auth C]|Fusion glycoprotein F0|Human parainfluenza 3 virus (strain NIH 47885) (11217) QIDITKLQHVGVLVNSPKGMKISQNFETRYLILSLIPKIEDSNSCGDQQIKQYKRLLDRLIIPLYDGLKLQKDVIVTNQESNENTDPRTERFFGGVIGTIALGVATSAQITAAVALVEAKQAKSDIEKLKEAIRDTNKAVQSVCSSVGNCIVAIKSVQDYVNKEIVPSIARLGCEAAGLQLGIALTQHYSELTNCFGDNIGSLQEKGIKLQCIASLYRTNITEIFTTSTVDKYDIYDLLFTESIKVRVIDVDLNDYSITLQVRLPLLTRLLNTQIYKVDSISYNIQNREWYIPLPSHIMTKGAFLGGADVKECIEAFSSYICPSDPGFVLNHEMESCLSGNISQCPRTTVTSDIVPRYAFVNGGVVANCITTTCTCNGIGNRINQPPDQGVKIITHKECNTIGINGMLFNTNKEGTLAFYTPDDITLNNSVALDPIDISIELNKVKSDLEESKEWYRRSNQKLSAIEDKIEEILSKIYHIENEIARIKKLIGEAP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASP50 HIS52 SER53 GLY55 PHE56 ASN58 ARG95 LEU99 L: ARG29 THR30 TYR31 TYR32 ARG91 ARG93 SER94 B: GLN139 SER142 ASP143 GLU145 LYS146 LEU147 GLU149 ALA150 ARG152 ASP153 THR154 GLN159 SER160 VAL161 CYS162 SER163 SER164 VAL165 ILE169 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
