Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5987 |
| PDBID: | 8DFL |
| Chains: | F_B |
| Organism: | Homo sapiens, Aequorea victoria, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.25 |
| Reference: | 10.1038/s41467-022-31285-5 |
| Antibody | |
| Antibody: | A0194009G09 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-activated potassium (Kv) channel 1.3 |
| Antigen mutation: | No |
| Durg Target: | P22001 |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >8DFL_F|Chain B[auth E], C[auth F], D[auth G], E[auth H]|Nanobody A0194009G09|Lama glama (9844) MEVQLVESGGGLVQAGGSLGLSCSASGLLFSRNSAGWYRQAPGKQREFVARIRMGGSINYADTVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCSSWRTGFYEYWGQGTLVTVSSAAAHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >8DFL_B|Chain A, F[auth B], G[auth C], H[auth D]|Potassium voltage-gated channel subfamily A member 3,Green fluorescent protein fusion|Homo sapiens (9606) MDERLSLLRSPPPPSARHRAHPPQRPASSGGAHTLVNHGYAEPAAGRELPPDMTVVPGDHLLEPEVADGGGAPPQGGCGGGGCDRYEPLPPSLPAAGEQDCCGERVVINISGLRFETQLKTLCQFPETLLGDPKRRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRIRRPVNVPIDIFSEEIRFYQLGEEAMEKFREDEGFLREEERPLPRRDFQRQVWLLFEYPESSGPARGIAIVSVLVILISIVIFCLETLPEFRDEKDYPASTSQDSFEAAGNSTSGSRAGASSFSDPFFVVETLCIIWFSFELLVRFFACPSKATFSRNIMNLIDIVAIIPYFITLGTELAERQGNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPTSGFSSIPDAFWWAVVTMTTVGYGDMHPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQSQYMHVGSCQHLSSSAEELRKARSNSTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDVSLEVLFQGPAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSAWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: VAL3 GLY27 LEU28 LEU29 ARG32 ASN33 MET54 ARG100 PHE103 TYR104 TYR106 B: TYR417 PHE418 ALA421 ASP422 ASP423 PRO424 THR425 SER426 GLY427 PHE428 SER429 MET450 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
