Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5973 |
| PDBID: | 8DF1 |
| Chains: | MN_B |
| Organism: | Homo sapiens, Oryctolagus cuniculus |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1093/abt/tbac027 |
| Antibody | |
| Antibody: | C59 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human chitinase 3-like-1 (hChi3l1) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >8DF1_M|Chain G[auth H], I[auth M], K[auth O], M[auth Q], O[auth S], Q[auth U]|C59 Fab Heavy chain|Oryctolagus cuniculus (9986) QSVEESGGDLVKPGASLTLTCTASGFTISSDYYMCWVRQAPGKGLEWIGCIYIGSGTDTYYASWAKGRFTISKTSSTTVTLQMTSLTAADTATYFCARDKGWSNAWGFYFQLWGPGTLVTVSSGQPKAPSVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSGTLTNGVRTFPSVRQSSGLYSLSSVVSVTSSSQPVTCNVAHPATNTKVDKTVAPSTC |
Light Chain: N
Mutation: NULL
| >8DF1_N|Chain H[auth L], J[auth N], L[auth P], N[auth R], P[auth T], R[auth V]|C59 Fab Light chain|Oryctolagus cuniculus (9986) DPVLTQTPASVEVPVGGTVTINCQASQSIGKYLNWYQQKPGQPPKLLIYSSSSLASGVSSRFKGSGFGTQFTLTISGVQCADAATYYCQQGYSYVDLENGFGGGTELEILGDPVAPTVLIFPPAADQVATGTVTIVCVANKYFPDVTVTWEVDGTTQTTGIENSKTPQNSADCTYNLSSTLTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRGDC |
Antigen
Chain: B
Mutation: NULL
| >8DF1_B|Chain A, B, C, D, E, F|Chitinase-3-like protein 1|Homo sapiens (9606) YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: ASP32 TYR34 ASP56 TYR58 TRP98 N: ASP1 GLN27 SER28 ILE29 GLY30 LYS31 TYR32 PHE67 THR69 TYR92 SER93 TYR94 B: HIS70 LYS89 ARG122 LYS126 SER127 PRO129 PRO130 PHE131 ARG133 THR134 HIS135 GLY136 PHE137 ASP138 LYS168 GLU169 ALA170 GLN171 PRO172 GLY173 LYS174 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
