Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5954 |
| PDBID: | 8DB4 |
| Chains: | IJ_F |
| Organism: | Homo sapiens, Arachis hypogaea |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1172/JCI164501 |
| Antibody | |
| Antibody: | 22S1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Arachis hypogaea 2 immunodominant allergen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >8DB4_I|Chain C, I|22S1 Heavy chain|Homo sapiens (9606) QVQLVQSGGGLVKPGGSLRLSCTASGFSFSDSYMSWIRQAPGKGLEWLTYISGSGEIISYADSVKGRFTISRDNAKKSVYLQMDSLRAEDTAVYYCARPSDYFETSEELDWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTH |
Light Chain: J
Mutation: NULL
| >8DB4_J|Chain D, J|22S1 Light chain|Homo sapiens (9606) EIVMTQSPSSLSASVGDRVTITCRASQSISTYLNWYQQKPGKAPNLLIYAASSLHSGVPSRFRGSGSGTDFTLTITSLQPDDFATYYCHQSYSAPRTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: F
Mutation: NULL
| >8DB4_F|Chain E, F|Ara h 2 allergen|Arachis hypogaea (3818) AARRCQSQLERANLRPCEQHLMQKIQRDEDSYERDPYSPSQDPYSPSPYDRRGAGSSQHQERCCNELNEFENNQRCMCEALQQIMENQSDRLQGRQQEQQFKRELRNLPQQCGLRAPQRCDLDVESGGRDRY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: SER28 SER30 ASP31 TYR33 SER54 ILE57 SER100 ASP101 TYR102 PHE103 GLU104 THR105 SER106 GLU107 J: SER28 SER30 THR31 TYR32 ASN34 TYR49 ALA50 HIS55 SER56 SER91 TYR92 SER93 F: ASN100 GLN102 LEU109 ARG131 ARG134 ASN135 GLN138 GLN139 ARG143 ALA144 PRO145 GLN146 ARG147 CYS148 LEU150 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
