Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5952 |
| PDBID: | 8DAM |
| Chains: | C_A |
| Organism: | Mus musculus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1038/s41467-022-35025-7 |
| Antibody | |
| Antibody: | E8 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mus musculus voltage-dependent L-type calcium channel subunit beta-1 |
| Antigen mutation: | No |
| Durg Target: | Q02641; Q02641; |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >8DAM_C|Chain C|Nanobody E8|Lama glama (9844) GQVQLQESGGGSVQAGGSLRLSCAASGRTFSKNAMGWFRQAPGKEREFVVAISWSGRNTYYADSVKGRFTISRDNAKNTVDLQMNSLKPEDSAVYYCAVGGDWRVYDISFYYTAHQYEYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >8DAM_A|Chain A|Voltage-dependent L-type calcium channel subunit beta-1|Mus musculus (10090) GSAESYTSRPSDSDVSLEEDREALRKEAERQALAQLEKAKTKPVAFAVRTNVGYNPSPGDEVPVQGVAITFEPKDFLHIKEKYNNDWWIGRLVKEGCEVGFIPSPVKLDSLRLLQEQTLRQNRLSSSKSGDNSSSSLGDVVTGTRRPTPPASAKQKQKSTEHVPPYDVVPSMRPIILVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKHIIIERSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPIIVYIKITSPKVLQRLIKSRGKSQSKHLNVQIAASEKLAQCPPEMFDIILDENQLEDACEHLAEYLEAYWKATHPPSSTPPNPLLNRT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: LYS32 SER53 TRP54 SER55 ARG57 ASN58 TYR60 LYS66 TRP103 ARG104 VAL105 TYR106 ASP107 ILE108 PHE110 TYR111 HIS115 GLN116 A: ASN108 VAL109 GLY110 TYR111 ASN112 PRO113 SER114 GLY116 ASP117 THR127 PHE128 GLU129 PRO130 LYS131 ASP132 LEU149 LYS151 LEU165 LEU168 ARG169 LEU171 GLN172 LEU176 GLN178 HIS219 GLU383 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
