Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5947 |
| PDBID: | 8D7E |
| Chains: | FG_C |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.93 |
| Reference: | 10.1126/sciadv.ade4395 |
| Antibody | |
| Antibody: | H4H25311P2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human ciliary neurotrophic factor receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >8D7E_F|Chain C[auth F]|H4H25311P2 antibody Fab fragment heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCVASGFTFSTSVMHWVRQAPGKGLEWVANIWYDGINKFYVDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAREFWSAFDLWGQGTMVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVES |
Light Chain: G
Mutation: NULL
| >8D7E_G|Chain B[auth G]|H4H25311P2 antibody Fab fragment light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >8D7E_C|Chain A[auth C]|Ciliary neurotrophic factor receptor subunit alpha|Homo sapiens (9606) QRHSPQEAPHVQYERLGSDVTLPCGTANWDAAVTWRVNGTDLAPDLLNGSQLVLHGLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNTFNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNATAITFDEFTIVKPDPPENVVARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQVAAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAAETTTSTTSSLAPPPTTKICDPGELGSEQKLISEEDLGGEQKLISEEDLHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: SER30 SER31 TYR33 TYR50 GLY51 SER54 ARG55 THR57 TYR92 GLY93 PRO96 TRP97 F: TRP47 ASN50 TRP52 TYR53 ASP54 ASN57 LYS58 PHE59 PHE100 TRP101 SER102 C: GLU111 THR140 ASN142 THR144 LEU146 GLY148 SER149 ILE151 LYS178 SER180 SER182 SER184 ASN185 ALA186 LEU187 GLY188 HIS189 ASN190 ALA191 THR192 ALA193 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
