Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5938 |
| PDBID: | 8CZ5 |
| Chains: | HL_A |
| Organism: | Lagovirus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.65 |
| Reference: | 10.1128/jvi.01217-22 |
| Antibody | |
| Antibody: | 2D9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rabbit hemorrhagic disease virus GI.2 (N11 strain) protruding domain (RHDV N11 P domain) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8CZ5_H|Chain B[auth H]|Fab 2D9 Heavy Chain|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFTKYGLNWVRQAPGKGLKWMGWIDTYTGEPTYADDFKGRFAFSLETSANTAYLQINYLKDEDMATYFCTRYDYGEGSGPQFTYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: L
Mutation: NULL
| >8CZ5_L|Chain C[auth L]|Fab 2D9 Light Chain|Mus musculus (10090) ELVMTQTPASLSVSVGETVTITCRASDNIYSNLAWYQQKQGKSPQLLVFAATNLADGVPSRFSGSGSGTQYSLKINSLQSEDFGNYYCQHFWGIPWTFGGGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >8CZ5_A|Chain A|N11 P domain|Lagovirus (95339) ISPADLLTTPVLTGVGTDNRWNGEIVGLQPVPGGFSTCNRHWNLNGSTFGWSSPRFAAIDHDRGNASYPGSSSSNVLELWYASAGSAADNPISQIAPDGFPDMSFVPFSGTTVPTAGWVGFGGIWNSSNGAPFVTTVQAYELGFATGAPSNPQPTTTTSGAQIVAKSIYGVATGINQATAGLFVMASGVISTPNSSAITYTPQPNRIVNAPGTPAAAPIGKNTPIMFASVVRRTGDINAEAGSTNGTQYGAGSQPLPVTVGLSLNNYSSALMPGQFFVWQLNFASGFMELGLSVDGYFYAGTGASATLIDLSELVDIRPVGPRPSTSTLVYNL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 LYS31 TYR32 GLY33 ASN35 TRP50 ASP52 THR53 TYR54 THR55 GLU57 PRO58 THR59 TYR60 ASP62 LYS65 TYR99 TYR101 GLY102 GLU103 GLY104 L: LEU2 TYR30 ASN32 PHE91 TRP92 GLY93 ILE94 TRP96 A: ARG299 GLY300 ASN301 ALA302 SER303 TYR304 PRO305 GLY306 SER307 LEU313 LEU315 TYR317 SER319 PRO343 PHE344 SER345 GLY346 THR382 GLY383 ALA384 PRO385 SER386 ASN387 PRO388 GLN389 SER395 GLY396 ALA397 GLN398 LYS402 ASN412 GLN413 ALA414 THR415 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
