Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 590 |
| PDBID: | 3NFP |
| Chains: | AB_K |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.86 |
| Reference: | 10.1038/cr.2010.130 |
| Antibody | |
| Antibody: | daclizumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Daclizumab(Approved) |
| Antigen | |
| Antigen: | Interleukin-2 receptor subunit alpha(CD25) |
| Antigen mutation: | No |
| Durg Target: | P01589 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >3NFP_A|Chain A, C[auth H]|Heavy chain of Fab fragment of daclizumab|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGYTFTSYRMHWVRQAPGQGLEWIGYINPSTGYTEYNQKFKDKATITADESTNTAYMELSSLRSEDTAVYYCARGGGVFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: B
Mutation: NULL
| >3NFP_B|Chain B, D[auth L]|Light chain of Fab fragment of daclizumab|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCSASSSISYMHWYQQKPGKAPKLLIYTTSNLASGVPARFSGSGSGTEFTLTISSLQPDDFATYYCHQRSTYPLTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: K
Mutation: NULL
| >3NFP_K|Chain E[auth I], F[auth K]|Interleukin-2 receptor subunit alpha|Homo sapiens (9606) ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER31 TYR32 ARG33 HIS35 TYR50 ASN52 THR55 TYR57 GLU59 TYR60 GLN62 ARG98 GLY100 GLY101 VAL102 B: ASP1 SER30 TYR31 HIS33 TYR48 THR49 ASN52 ARG90 SER91 THR92 TYR93 PRO94 LEU95 K: GLU1 LEU2 CYS3 ASP4 ASP5 ASP6 GLY23 MET25 ASN27 LEU42 TYR43 LEU45 ILE118 HIS120 VAL122 MET149 THR150 HIS151 GLY152 LYS153 THR154 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
