Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5899 |
| PDBID: | 8CWJ |
| Chains: | JK_G |
| Organism: | Homo sapiens, Pangolin coronavirus |
| Method: | XRD |
| Resolution (Å): | 2.45 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | CR3022 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Pangolin coronavirus spike glycoprotein RBD(pRBD) |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >8CWJ_J|Chain G[auth J], I[auth U]|Heavy chain of 4C12-B12 antibody Fab|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFRISDEDMGWVRQAPGKGLEWVSYIDNAGASTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKSHYANPSFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGSHHHHHH |
Light Chain: K
Mutation: NULL
| >8CWJ_K|Chain H[auth K], J[auth V]|Light chain of 4C12-B12 antibody Fab|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDIADYLNWYQQKPGKAPKLLIYYASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGLGMPITFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECS |
Antigen
Chain: G
Mutation: NULL
| >8CWJ_G|Chain C[auth G], F[auth I]|Spike glycoprotein|Pangolin coronavirus (2708335) TNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVRGDEVRQIAPGQTGRIAGYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFHPTNGVGYQPYRVVVLSFELLNAPATVCGPKGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: ARG28 ASP31 TYR50 ASP52 ASN53 ALA54 GLY55 SER57 TYR59 HIS100 TYR101 ALA102 ASN103 PRO104 K: ASP1 ILE2 GLN27 ASP28 ALA30 ASP31 TYR32 TYR50 SER67 LEU92 GLY93 MET94 PRO95 G: THR346 PHE347 ALA348 SER349 TYR351 ALA352 TRP353 ASN354 ARG355 LYS356 ARG357 SER359 ASN360 ASN394 TYR449 ASN450 LEU452 PHE464 GLU465 ARG466 ASP467 ILE468 SER469 THR470 ILE472 GLY482 GLU484 PHE490 LEU492 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
