Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5898 |
| PDBID: | 8CWJ |
| Chains: | HL_G |
| Organism: | Homo sapiens, Pangolin coronavirus |
| Method: | XRD |
| Resolution (Å): | 2.45 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 4C12-B12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Pangolin receptor binding domain (pRBD) |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8CWJ_H|Chain A, D[auth H]|Heavy chain of CR3022 antibody Fab|Homo sapiens (9606) QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGSHHHHHH |
Light Chain: L
Mutation: NULL
| >8CWJ_L|Chain B, E[auth L]|Light chain of CR3022 antibody Fab|Homo sapiens (9606) DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: G
Mutation: NULL
| >8CWJ_G|Chain C[auth G], F[auth I]|Spike glycoprotein|Pangolin coronavirus (2708335) TNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVRGDEVRQIAPGQTGRIAGYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFHPTNGVGYQPYRVVVLSFELLNAPATVCGPKGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 GLY28 ILE30 THR31 TYR32 TRP33 TYR52 GLY54 ASP55 SER56 GLU57 ARG59 LYS74 SER100 GLY101 ILE102 SER103 THR104 PRO105 ASP107 L: TYR31 SER32 SER33 ILE34 ASN35 LYS36 TYR38 TYR55 TRP56 GLU61 SER62 TYR98 G: TYR369 ASN370 SER371 PHE374 SER375 THR376 PHE377 LYS378 CYS379 TYR380 GLY381 VAL382 SER383 PRO384 THR385 LYS386 LEU390 PHE392 ARG408 PRO412 GLN414 ASP427 ASP428 PHE429 THR430 PHE515 GLU516 LEU517 LEU518 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
