Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5895 |
| PDBID: | 8CW9 |
| Chains: | NO_F |
| Organism: | human metapneumovirus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.46 |
| Reference: | 10.1016/j.immuni.2022.07.003 |
| Antibody | |
| Antibody: | MPE8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prefusion-stabilized human metapneumovirus (hMPV) fusion protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: N
Mutation: NULL
| >8CW9_N|Chain G, J, N|MPE8 heavy chain|Homo sapiens (9606) EVQLVESGGGLVKPGGSLRLSCAASGFTFSSYSMNWVRQAPGKGLEWVSSISASSSYSDYADSAKGRFTISRDNAKTSLFLQMNSLRAEDTAIYFCARARATGYSSITPYFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
Light Chain: O
Mutation: NULL
| >8CW9_O|Chain B, M, O|MPE8 light chain|Homo sapiens (9606) QSVVTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYDNNNRPSGVPDRFSASKSGTSASLAITGLQAEDEADYYCQSYDRSLSGVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: F
Mutation: Q100R/S101R/L110C/T127C/A140C/A147C/N153C/A185P/L219K/V231I/N322C/T365C/E453Q/V463C
| >8CW9_F|Chain A, E, F|Fusion glycoprotein F0|Human metapneumovirus (162145) MSWKVVIIFSLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRRRRFVLGAIACGVATAAAVTAGVAIAKCIRLESEVTAIKNCLKKTNECVSTLGCGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISKDLMTDAELARAISNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPCEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSCGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPQDQFNVALDQCFESIENSQALVDQSNRILSSAEKGNTGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: THR28 SER30 SER31 TYR32 SER52 ALA53 SER54 SER56 TYR57 ASP59 ARG100 ALA101 THR102 GLY103 TYR104 SER105 SER106 ILE107 O: GLY30 ALA31 GLY32 TYR33 LYS68 GLY70 TYR93 ARG95 F: LEU36 THR41 GLY42 TRP43 TYR44 ALA120 ALA123 ILE124 THR150 VAL155 ARG156 SER232 ASN233 MET234 PRO235 THR236 SER237 ALA238 GLY239 GLN240 ILE241 LEU243 ILE275 GLY277 VAL278 ILE279 ASP280 THR281 PRO282 GLN312 ALA314 GLY315 HIS332 GLU345 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
